Map1lc3b (NM_026160) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG200654
Map1lc3b (tGFP-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b)
$489.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Map1lc3b |
| Synonyms | 1010001C15Rik; Atg8; LC3b; Map1lc3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_026160 |
| ORF Size | 375 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_026160.5 |
| RefSeq Size | 1711 bp |
| RefSeq ORF | 378 bp |
| Locus ID | 67443 |
| UniProt ID | Q9CQV6 |
| Cytogenetics | 8 E1 |
| Summary | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_026160" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC203443 | Map1lc3b (untagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10ug) | 10 ug |
$150.00
|
|
| MR200654 | Map1lc3b (Myc-DDK-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b) | 10 ug |
$289.00
|
|
| MR200654L3 | Lenti ORF clone of Map1lc3b (Myc-DDK-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b) | 10 ug |
$450.00
|
|
| MR200654L4 | Lenti ORF clone of Map1lc3b (mGFP-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b) | 10 ug |
$450.00
|
|
Citations
Autophagy induction promotes renal cyst growth in polycystic kidney disease
Lee, Ko, Oh et al
EBioMedicine (2020) 60, 102986 DOI: 10.1016/j.ebiom.2020.102986
Lee, Ko, Oh et al
EBioMedicine (2020) 60, 102986 DOI: 10.1016/j.ebiom.2020.102986
Cited by 21
AI Summary: In this study, OriGene's GFP-tagged LC3 plasmid was used to monitor and analyze autophagy processes in cell models of polycystic kidney disease, aiding the investigation of how autophagy regulation affects renal cyst growth.
Snippet: iMAX (Invitrogen, Carlsbad, CA, USA). Fugene (Promega, Fitchburg, WI, USA) was used for transfection of plasmids including RFP-GFP-LC3 (Thermo Fisher Scientific, Waltham, MA, USA) and GFP-tagged Lc3 (OriGene Technologies, Rockville, MD, USA) according to the manufacturer's instructions. Drugs used to regulate autophagy were rapamycin, 3-methyladenine (3-MA), and chloroquine (CQ) (Sigma-Aldrich, St...
Cross-talk between miR-471-5p and autophagy component proteins regulates LC3-associated phagocytosis (LAP) of apoptotic germ cells
Panneerdoss, Viswanadhapalli, Abdelfattah et al
Nat Commun (2017) 8 (1), 598 DOI: 10.1038/s41467-017-00590-9
Panneerdoss, Viswanadhapalli, Abdelfattah et al
Nat Commun (2017) 8 (1), 598 DOI: 10.1038/s41467-017-00590-9
AI Summary: OriGene products contributed to this study by providing MAP1LC3B (cat #MG200654) and ELMO1 (cat #MR206637) plasmids, which were used to investigate the molecular mechanisms underlying LC3-associated phagocytosis in Sertoli cells.
Snippet: MAP1LC3B and ELMO1 plasmids were purchased from Origene (MAP1LC3B;cat #MG200654;ELMO1 cat #MR206637)
Showing 1-2 of 2 papers.
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