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Rprd1b (NM_027434) Mouse Untagged Clone

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SKU
MC201990
Rprd1b (untagged) - Mouse regulation of nuclear pre-mRNA domain containing 1B (Rprd1b), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Rprd1b
Synonyms 2610304G08Rik; 2810446G03Rik; Crept
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC028819 sequence for NM_027434
CGGACGCGTGGGGCGCGGGCGGCTGTTACTGCGGAGACCTGTCCCCTCCCCCCACCTGGTACCCCGAGCT GTCTGTCAGTCGGCGGAGAGCCCTGCAGCCTGTCAGCGGAGGCCTGGGCCGAGTGCGGTCGCTTTCCCCG TCTCATCGGGCGGCCCGGGCTGCTCCCCACCACCGCCACCATGTCCTCCTTCTCAGAATCGGCGCTGGAA AAGAAGCTCTCAGAGCTGAGCAACTCTCAGCAGAGCGTGCAGACCCTCTCCCTGTGGCTCATCCACCACC GCAAGCACGCAGGACCCATCGTCTCCGTGTGGCACCGCGAGCTCCGCAAAGCCAAATCAAATAGAAAGCT TACTTTTCTATACTTAGCAAATGATGTCATCCAAAACAGTAAAAGGAAAGGACCTGAGTTCACAAGAGAA TTTGAGTCTGTGCTTGTGGATGCTTTTTCTCATGTTGCCAGAGAGGCAGATGAAGGCTGTAAAAAACCTT TAGAAAGATTGCTGAACATCTGGCAAGAACGAAGTGTGTACGGCGGCGAGTTCATACAGCAGCTGAAGCT GTCTATGGAGGACTCCAAGAGCCCTCCCCCCAAAGCAGCAGAAGAGAAGAAGTCTCTAAAACGAACTTTT CAGCAGATACAAGAGGAGGAAGATGATGACTACCCTGGAAGCTACTCTCCCCAAGACCCTTCTGCAGGCC CTCTCTTGACTGAGGAGTTAATCAAAGCTTTGCAGGATCTGGAAAATGCTGCGTCAGGGGATGCTACTGT CCGACAGAAGATCGCTTCCCTGCCTCAGGAAGTGCAAGACGTGTCGCTGCTAGAGAAAATTACAGACAAA GAGGCAGCTGAACGTCTTTCAAAAACAGTAGATGAAGCATGTCTGTTACTAGCGGAATATAACGGGCGCC TGGCAGCGGAACTGGAAGACCGGCGCCAGCTGGCTCGGATGCTGGTGGAATACACCCAGAACCAGAAAGA GGTTTTGTCAGAAAAAGAGAAAAAACTAGAAGAGTATAAACAGAAGCTTGCTCGAGTAACCCAGGTCCGC AAGGAACTCAAGTCCCACATTCAGAGCTTGCCAGACCTTTCGCTGTTGCCTAATGTCACAGGGGGCCTGG CACCTCTGCCCTCTGCTGGTGACCTCTTTTCAACTGACTAGGACAGGTATCATGCCCCAGGTTCCCATGT GTGCCAGAGAAAAGAAGCAAGTCACTGCTGGAGACAGTCCCCTGCCCCAGCTCCATCTCTTCAGCCAGCA TCTGCCTCAGTAATGAAGCGGGGAACTCTGACTTCTTACTCTCCTCCGGCTCCCTCTGAGCATGGTTCAG AGCCATGCAGATGGACTCAGTTTTGATTCATATTTGCTAAGAACACAGACTCTCCCTCCCACTCATGTCT CTTGCCACTGCTGCTTTTCCATCTTTCGCTCTCTTACACCCATAAGCCATGAAAATCATGTGTACTTCTG GAAGCTTTCAAGAGAGGCTTGCCTCAGTGACAGCACATGCCATGGAAGTCTGAGCTGGCCTTGACCAGGT TCAGAACAGGCTCCCAATGAGGACTCAGGCTGCAGGGATTGGGCTTTCCTGGCCACCATTGCTCACAAGG TCGGCAGCATAGCTTTGCCACAAAGAGTTTGCCAAATTGCCGACATTTCCTGATCTAAACACTTCTGTTA GAGAAGTGTTGTCATGGTTAGGTGTGTTTATTTTTCGAGCATTCTAGAGAATCCAACAGTAAGATTCGAA GCTGATCTGAGACAAAGTTATAGCGCAGGTACAGAGTGGCAGGGCTCACAAGTCATTTGCCAGGCTTCTC ACCACAGCCTTCCCTGCCACATTGCTCCAGGGTCCCCAGTTTCAGAGCACCTTCCTAAAGCAGGACCCTC TGTCCCCTGGGGCTGGGAAGGGAGCTGTTGGGATAGCATGCCCAGGCTGATAACTGCTCCACAGCTGGAG TCTGGGCTCTCGTGCATGTTGCTCTCACCCCGAAGCTTACAATAACGCCACCATGTGGACGCATCCACAG CATGGTGACTGGACTTGAACATCTCTACAGATAGGACACTGAGCGTGCCAGGCAGTTCCTGCCACTATAT GCATCCTTGCTCCCATGCAGAGAGAGGAGTACTCAAACAACTCACCTCCAGCAGAGGAAGGGAGTGGAGG GAGACTAATTTGGGGTTGAATTCCATTATCATGCCAGCTGACATTATGACTGAATGATGTAGTTGCGATT TTTACGTCTAGTAAAGAGTGGGCCTGGCCCTGGAAATGACTGTAATCTGGCAGGCTTATATTTGACATTG GAAAGGGCAGAAACCATTTTGTCCTACTAGTGTGATAGGAATTGTCGCCCAGAGCTGAAGTCCAAAAGCC ATAAGAAGGAATTCAGTGAAGGGGCCTGAGAAGCTTCGTTGCTTTAAGTTGCTGTTTTCAGGATCACCAA AAAAAAAAGCACACATCGTTTTTACACGTTAAAGCAAATCTGTGTGTCACCAAATAGGCAGGCTTTGGTT GAAGAAGCCGCCCCACCTGCTATGAGATGGGACTCAGTTCCACTCTGTCCAGCCTGGTCTTCGTGGAAGC TGTTATTCACAGAGTGCAGGCTGCTGTTTGCTTGTTCACTCTGCCTTTTCTGTCAGCATCCTTTTCTCCA GAGGTTACAGGACAGCACTGACTCAGGTGAAACCCCTGTCTCTGCTGCGGAACACCTCCCTACACACCAC GCCACTTTACTGTCACATGCACACACCACTCTGAACACCCTCCAGACACTGCTTTCCCACAAAACAGACC AAAAGACTGTAGCAGCTCTGCTCTGATGACAACTTAGTGGGGTCAGCTCAATGGTGTTTATTCTTTGGAG TCTCTGACCAGCAATCAGTGCGTAACTCTTACAGCAGGATCAAGACGTGACCAGTAGCGATTATGTAAAT GAATGTGTTGGCCTCATTACACCACTTCCAGCAGATTTCCTGTGAGGAAGTTAGTTTCGTTTTAATGAAA TGCTTCAGTTTTTAATCTTCCTTGTTGTGTGCCTCCACCATGCCCCCAGTCTCCCAACTGTGTGCGAACT TCCCTTGTTTAACTTAAGTGAAACTTTTGTCCCTGAGAGTTGATCTGGCGGGTGGGGTGGGTGACTTCTG GTTTTGTGTTTCTTTAGGGCATCCGTAGGCCTCAAGGATCTTTCCTTTAGGGTCATATTCCTCAGAAAGT CTTCAGTCTTTCTTTGTTTTTGTTTTGTTTTTCTTAAAGGATATTTTTAAAGCTTAAATTTGTATATTAA TTTAGGACTTTATTTAGAAGTATAGGCTGTCATTGGTGGCAGCAGTATATTCTGAAATGTCTCATAGATA TATATTTTTGAATAAAGATGGTGTTGTTGAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_027434
Insert Size 981 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028819, AAH28819
RefSeq Size 3490 bp
RefSeq ORF 981 bp
Locus ID 70470
UniProt ID Q9CSU0
Cytogenetics 2 H1
Summary Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.
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"NM_027434" in other vectors (4)
SKU Description Size Price
MG204728 Rprd1b (tGFP-tagged) - Mouse RIKEN cDNA 2610304G08 gene (2610304G08Rik) 10 ug
$500.00
MR204728 Rprd1b (Myc-DDK-tagged) - Mouse regulation of nuclear pre-mRNA domain containing 1B (Rprd1b) 10 ug
$289.00 $300.00
MR204728L3 Lenti ORF clone of Rprd1b (Myc-DDK-tagged) - Mouse regulation of nuclear pre-mRNA domain containing 1B (Rprd1b) 10 ug
$600.00
MR204728L4 Lenti ORF clone of Rprd1b (mGFP-tagged) - Mouse regulation of nuclear pre-mRNA domain containing 1B (Rprd1b) 10 ug
$600.00

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