RAB11FIP3 (NM_001142272) Human Tagged ORF Clone

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SKU
RC227246
RAB11FIP3 (Myc-DDK-tagged)-Human RAB11 family interacting protein 3 (class II) (RAB11FIP3), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RAB11FIP3
Synonyms CART1; FIP3-Rab11; Rab11-FIP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227246 representing NM_001142272
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTTCCTGAAGGCCAACGAGGTGACGGACAGCGCGTACATGGGCTCCGAGAGCACCTACAGTGAGT
GTGAGACCTTCACGGACGAGGACACCAGCACCCTGGTGCACCCTGAGCTGCAACCTGAAGGGGACGCAGA
CAGTGCCGGCGGCTCGGCCGTGCCCTCTGAGTGCCTGGACGCCATGGAGGAGCCCGACCATGGTGCCCTG
CTGCTGCTCCCAGGCAGGCCTCACCCCCATGGCCAGTCTGTCATCACGGTGATCGGGGGCGAGGAGCACT
TTGAGGACTACGGTGAAGGCAGTGAGGCGGAGCTGTCCCCAGAGACCCTATGCAACGGGCAGCTGGGCTG
CAGTGACCCCGCTTTCCTCACGCCCAGTCCGACAAAGCGGCTCTCCAGCAAGAAGGTGGCAAGGTACCTG
CACCAGTCAGGGGCCCTGACCATGGAGGCCCTGGAGGACCCTTCCCCCGAGCTCATGGAGGGCCCAGAGG
AGGACATTGCTGACAAGGTTGTCTTCCTGGAAAGGCGTGTGCTGGAGCTGGAAAAGGACACGGCAGCCAC
CGGTGAGCAACACAGCCGCCTGAGGCAGGAGAACCTGCAGCTGGTGCACAGAGCAAACGCCCTGGAGGAG
CAGCTGAAGGAGCAGGAGCTGAGAGCCTGCGAGATGGTCCTGGAAGAGACCCGGCGTCAGAAGGAGCTCC
TGTGCAAGATGGAGAGGGAGAAGAGCATTGAGATCGAGAACCTGCAGACCAGGCTACAGCAACTGGACGA
GGAGAACAGTGAACTCCGGTCCTGCACGCCCTGTCTGAAGGCCAACATTGAGCGTCTGGAGGAGGAGAAG
CAGAAGCTGTTGGATGAGATAGAGTCGCTGACGCTGCGGCTCAGTGAAGAGCAGGAGAACAAGAGGAGAA
TGGGGGACAGGCTGAGTCACGAGAGGCACCAGTTCCAGAGGGACAAGGAGGCCACCCAGGAGCTGATCGA
GGACCTCCGAAAGCAGCTGGAGCACCTGCAGCTCCTCAAGCTGGAGGCCGAGCAGCGGCGGGGCCGCAGC
AGCAGCATGGGCCTGCAGGAGTACCACAGCCGCGCCCGGGAGAGCGAGCTGGAGCAGGAGGTCCGCAGGC
TGAAGCAGGACAACCGCAACCTGAAGGAGCAGAACGAGGAGCTGAACGGGCAGATCATTACCCTCAGCAT
CCAGGGCGCCAAGAGCCTCTTCTCCACAGCCTTCTCTGAGTCCCTGGCTGCAGAGATCAGCTCCGTCTCC
CGAGATGAGCTCATGGAGGCGATTCAGAAGCAGGAGGAGATCAACTTCCGCCTGCAGGACTACATCGACA
GGATCATCGTGGCCATCATGGAGACCAACCCGTCCATCCTGGAGGTCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC227246 representing NM_001142272
Red=Cloning site Green=Tags(s)

MPFLKANEVTDSAYMGSESTYSECETFTDEDTSTLVHPELQPEGDADSAGGSAVPSECLDAMEEPDHGAL
LLLPGRPHPHGQSVITVIGGEEHFEDYGEGSEAELSPETLCNGQLGCSDPAFLTPSPTKRLSSKKVARYL
HQSGALTMEALEDPSPELMEGPEEDIADKVVFLERRVLELEKDTAATGEQHSRLRQENLQLVHRANALEE
QLKEQELRACEMVLEETRRQKELLCKMEREKSIEIENLQTRLQQLDEENSELRSCTPCLKANIERLEEEK
QKLLDEIESLTLRLSEEQENKRRMGDRLSHERHQFQRDKEATQELIEDLRKQLEHLQLLKLEAEQRRGRS
SSMGLQEYHSRARESELEQEVRRLKQDNRNLKEQNEELNGQIITLSIQGAKSLFSTAFSESLAAEISSVS
RDELMEAIQKQEEINFRLQDYIDRIIVAIMETNPSILEVK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142272
ORF Size 1380 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142272.1, NP_001135744.1
RefSeq ORF 1341 bp
Locus ID 9727
UniProt ID O75154
Cytogenetics 16p13.3
Protein Pathways Endocytosis
MW 52.3 kDa
Summary Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP3 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 PubMed 11495908).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_001142272" in other vectors (4)
SKU Description Size Price
RC227246L3 Lenti ORF clone of Human RAB11 family interacting protein 3 (class II) (RAB11FIP3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC227246L4 Lenti ORF clone of Human RAB11 family interacting protein 3 (class II) (RAB11FIP3), transcript variant 2, mGFP tagged 10 ug
$757.00
RG227246 RAB11FIP3 (tGFP-tagged) - Human RAB11 family interacting protein 3 (class II) (RAB11FIP3), transcript variant 2 10 ug
$657.00
SC325958 RAB11FIP3 (untagged)-Human RAB11 family interacting protein 3 (class II) (RAB11FIP3), transcript variant 2 10 ug
$503.00

Plasmid Map for RC227246

Download sequence for

Cloning scheme for RC227246

Circular map for RC227246

file-alt Citations

Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells
Busatto, Song, Kim et al
J Extracell Vesicles (2025) 14 (1), e70038 DOI: 10.1002/jev2.70038
Cited by 19
AI Summary: OriGene’s human tagged ORF clones for Rab11fip3 (rc227246) and Rab11fip5 (rc206173) were used as plasmids to investigate how these proteins contribute to breast cancer-derived extracellular vesicle-induced changes in blood-brain barrier endothelial cell dynamics.
Snippet: nd electroporated using Cell Line Nucleofector Kit V (Lonza Walkersville, USA). The cell pellet was resuspended in 100 µL of buffer, mixed with 2 µg of Rab11fip3 (human tagged orf clone rc227246, Origene Technologies Inc.) and Rab11fip5 (human tagged orf clone rc206173, Origene Technologies Inc.) plasmids, and electroporated using the S‐05 Program of the Amaxa Biosystem Nucleofector. After,
FigureFigureFigure
Showing 1-1 of 1 papers.
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