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Nogo A (RTN4) (NM_007008) Human Tagged ORF Clone

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SKU
RC221080
RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Nogo A
Synonyms ASY; Nbla00271; Nbla10545; NI220/250; NOGO; NSP; NSP-CL; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221080 representing NM_007008
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGGTCAGAAGAAAAATTGGAAGGACAAGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGA
CTGGAGTGGTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAAC
AGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCT
ATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGT
TGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTT
CTTAGTTGATGATTTAGTTGATTCTCTGAAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCC
TTGTTTAATGGTCTGACACTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAAC
GGCATCAGGCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAAT
CCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221080 representing NM_007008
Red=Cloning site Green=Tags(s)

MDGQKKNWKDKVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQA
IQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGA
LFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007008
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007008.3
RefSeq Size 1808 bp
RefSeq ORF 600 bp
Locus ID 57142
UniProt ID Q9NQC3
Cytogenetics 2p16.1
Domains Reticulon
Protein Families Transmembrane
MW 22.2 kDa
Summary This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_007008" in other vectors (6)
SKU Description Size Price
RC221080L1 Lenti ORF clone of Human reticulon 4 (RTN4), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC221080L2 Lenti ORF clone of Human reticulon 4 (RTN4), transcript variant 3, mGFP tagged 10 ug
$600.00
RC221080L3 Lenti ORF clone of Human reticulon 4 (RTN4), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC221080L4 Lenti ORF clone of Human reticulon 4 (RTN4), transcript variant 3, mGFP tagged 10 ug
$600.00
RG221080 RTN4 (tGFP-tagged) - Human reticulon 4 (RTN4), transcript variant 3 10 ug
$489.00 $500.00
SC107922 RTN4 (untagged)-Human reticulon 4 (RTN4), transcript variant 3 10 ug
$300.00

Plasmid Map for RC221080

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221080
Circular map for RC221080

file-alt Citations

Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics
Ngo, Takechi, Sivakumar et al
Nat Chem (2026)
Cited by 2
AI Summary: OriGene contributed to this study by providing the Myc-DDK-tagged open reading frame clone of Homo sapiens reticulon 4 (RTN4, product RC221080), which was used for protein expression and subsequent chemoproteomic analysis.
Snippet: Wucherpfennig. The Myc-DDK-tagged open reading frame clone of Homo sapiens reticulon 4 (RTN4), transcript variant 3, was obtained from Origene (RC221080) and also subcloned into pRK5-C-FLAG via GateWay cloning according to the manufacturer’s protocol
Showing 1-1 of 1 papers.
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