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CD99L2 (NM_031462) Human Tagged ORF Clone

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SKU
RC207079
CD99L2 (Myc-DDK-tagged)-Human CD99 molecule-like 2 (CD99L2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CD99L2 or NM_031462"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD99L2
Synonyms CD99B; MIC2L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207079 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCCTGGCGCTCGGCGTTCCTTGTCTGCCTCGCTTTCTCCTTGGCCACCCTGGTCCAGCGAGGAT
CTGGGGACTTTGATGATTTTAACCTGGAGGATGCAGTGAAAGAAACTTCCTCAGTAAAGCAGCCATGGGA
CCACACCACCACCACCACAACCAATAGGCCAGGAACCACCAGAGCTCCGGCAAAACCTCCAGGTAGTGGA
TTGGACTTGGCTGATGCTTTGGATGATCAAGATGATGGCCGCAGGAAACCGGGTATAGGAGGAAGAGAGA
GATGGAACCATGTAACCACCACGACCAAGAGGCCAGTAACCACCAGAGCTCCAGCAAATACTTTAGGAAA
TGATTTTGACTTGGCTGATGCCCTGGATGATCGAAATGATCGAGATGATGGCCGCAGGAAACCAATTGCT
GGAGGAGGAGGTTTTTCAGACAAGGATCTTGAAGACATAGTAGGGGGTGGAGAATACAAACCTGACAAGG
GTAAAGGTGATGGCCGGTACGGCAGCAATGACGACCCTGGATCTGGCATGGTGGCAGAGCCTGGCACCAT
TGCCGGGGTGGCCAGCGCCCTGGCCATGGCCCTCATCGGTGCCGTCTCCAGCTACATCTCCTACCAGCAG
AAGAAGTTCTGCTTCAGCATTCAGCAGGGTCTCAACGCAGACTACGTGAAGGGAGAGAACCTGGAAGCCG
TGGTATGTGAGGAACCCCAAGTGAAATACTCCACGTTGCACACGCAGTCTGCAGAGCCGCCGCCGCCGCC
CGAACCAGCCCGGATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207079 protein sequence
Red=Cloning site Green=Tags(s)

MVAWRSAFLVCLAFSLATLVQRGSGDFDDFNLEDAVKETSSVKQPWDHTTTTTTNRPGTTRAPAKPPGSG
LDLADALDDQDDGRRKPGIGGRERWNHVTTTTKRPVTTRAPANTLGNDFDLADALDDRNDRDDGRRKPIA
GGGGFSDKDLEDIVGGGEYKPDKGKGDGRYGSNDDPGSGMVAEPGTIAGVASALAMALIGAVSSYISYQQ
KKFCFSIQQGLNADYVKGENLEAVVCEEPQVKYSTLHTQSAEPPPPPEPARI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031462
ORF Size 786 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031462.4
RefSeq Size 3736 bp
RefSeq ORF 789 bp
Locus ID 83692
UniProt ID Q8TCZ2
Cytogenetics Xq28
Protein Families Transmembrane
MW 28 kDa
Summary This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. provided by RefSeq, May 2010
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Documents
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"NM_031462" in other vectors (4)
SKU Description Size Price
RC207079L3 Lenti ORF clone of Human CD99 molecule-like 2 (CD99L2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC207079L4 Lenti ORF clone of Human CD99 molecule-like 2 (CD99L2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG207079 CD99L2 (tGFP-tagged) - Human CD99 molecule-like 2 (CD99L2), transcript variant 1 10 ug
$489.00 $500.00
SC111457 CD99L2 (untagged)-Human CD99 molecule-like 2 (CD99L2), transcript variant 1 10 ug
$300.00

Plasmid Map for RC207079

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207079
Circular map for RC207079

file-alt Citations

Loss-of-function variants in the CAPN1 activator CD99L2 cause X-linked spastic ataxia
Menden, Incebacak Eltemur, Demidov et al
Nat Commun (2026) 17 (1), 1698 DOI: 10.1038/s41467-026-69337-9
Cited by 3
AI Summary: In this study, OriGene's C-terminally myc-DDK-tagged CD99L2 construct (pCMV6-Entry, RC207079) was used to investigate the cellular localization and functional interactions of CD99L2 in the context of X-linked spastic ataxia.
Snippet: 6-tagged forms of the proteins were generated (BioCat, Heidelberg, Germany). A C-terminally myc-DDK-tagged CD99L2 construct carried by a pCMV6-Entry vector was additionally purchased (RC207079, OriGene). Ataxin-3 overexpression was performed using pcDNA3.1 vectors harbouring C-terminally V5-tagged constructs with 15 or 77 glutamines50. Overe
FigureFigureFigure
Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration
Bedau, Peters, Prox et al
FASEB J. (2016)
AI Summary: OriGene’s pCMV6-Entry mammalian expression vectors with C-terminal myc-DDK tag (RC204056 and RC207079) were used in this study to express human CD99, enabling investigation of its cleavage by meprin β and its role in transendothelial cell migration.
Snippet: to the manufacturer's instructions. For expression of human CD99 mammalian expression vector pCMV6-Entry with C-terminal myc-DDK tag (RC204056 and RC207079;Origene, Rockville, MD, USA) was used. Meprin b and ... Cite SaveSaving
Showing 1-2 of 2 papers.
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