RAB11FIP5 (NM_015470) Human Tagged ORF Clone
SKU
RC206173
RAB11FIP5 (Myc-DDK-tagged)-Human RAB11 family interacting protein 5 (class I) (RAB11FIP5)
$910.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RAB11FIP5 |
| Synonyms | gaf-1; GAF1; pp75; rab11-FIP5; RIP11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_015470 |
| ORF Size | 1956 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015470.3 |
| RefSeq Size | 4340 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 26056 |
| UniProt ID | Q9BXF6, Q9UFM0 |
| Cytogenetics | 2p13.2 |
| Protein Pathways | Endocytosis |
| MW | 70.2 kDa |
| Summary | Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_015470" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206173L1 | Lenti ORF clone of Human RAB11 family interacting protein 5 (class I) (RAB11FIP5), Myc-DDK-tagged | 10 ug |
$1,210.00
|
|
| RC206173L2 | Lenti ORF clone of Human RAB11 family interacting protein 5 (class I) (RAB11FIP5), mGFP tagged | 10 ug |
$1,210.00
|
|
| RC206173L3 | Lenti ORF clone of Human RAB11 family interacting protein 5 (class I) (RAB11FIP5), Myc-DDK-tagged | 10 ug |
$1,210.00
|
|
| RC206173L4 | Lenti ORF clone of Human RAB11 family interacting protein 5 (class I) (RAB11FIP5), mGFP tagged | 10 ug |
$1,210.00
|
|
| RG206173 | RAB11FIP5 (tGFP-tagged) - Human RAB11 family interacting protein 5 (class I) (RAB11FIP5) | 10 ug |
$1,110.00
|
|
| SC304319 | RAB11FIP5 (untagged)-Human RAB11 family interacting protein 5 (class I) (RAB11FIP5) | 10 ug |
$974.00
|
|
Citations
Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells
Busatto, Song, Kim et al
J Extracell Vesicles (2025) 14 (1), e70038 DOI: 10.1002/jev2.70038
Busatto, Song, Kim et al
J Extracell Vesicles (2025) 14 (1), e70038 DOI: 10.1002/jev2.70038
Cited by 19
AI Summary: OriGene’s human tagged ORF clones for Rab11fip3 (rc227246) and Rab11fip5 (rc206173) were used as plasmids to investigate how these proteins contribute to breast cancer-derived extracellular vesicle-induced changes in blood-brain barrier endothelial cell dynamics.
Snippet: nd electroporated using Cell Line Nucleofector Kit V (Lonza Walkersville, USA). The cell pellet was resuspended in 100 µL of buffer, mixed with 2 µg of Rab11fip3 (human tagged orf clone rc227246, Origene Technologies Inc.) and Rab11fip5 (human tagged orf clone rc206173, Origene Technologies Inc.) plasmids, and electroporated using the S‐05 Program of the Amaxa Biosystem Nucleofector. After,
Showing 1-1 of 1 papers.
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