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Prkg2 (NM_008926) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR225140
Prkg2 (Myc-DDK-tagged) - Mouse protein kinase, cGMP-dependent, type II (Prkg2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $699.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prkg2
Synonyms AW212535; cGK-II; CGKII; Prkgr2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR225140 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAATGGTTCAGTGAAGCCCAAGCATGCTAAGCACCCAGATGGCCATTCAGGGAACCTCAGCAATG
AAGCCCTGCGGAGCAAGGTGTTGGAGCTGGAGCGGGAGCTGAGGAGGAAGGACGCGGAACTTCAGGAACG
GGAGTACCACCTGAAGGAGCTTCGGGAGCAGCTGGCCAAGCAGACCGTGGCCATCGCAGAGCTCACAGAA
GAACTGCAGAGCAAGTGCATCCAGCTGAACAAGCTGCAGGATGTGATCCACGTGCAGGGAGGAAGCCCAC
TGCAGGCCTCTCCCGACAAAGTGCCTCTGGATGTTCACCGCAAGACCTCAGGCTTGGTCTCGCTCCACAG
CAGGAGGGGGGCGAAGGCTGGAGTGTCTGCAGAGCCAACCACCCGAACCTATGACCTCAACAAACCCCCC
GAGTTTTCCTTTGAGAAAGCGAGAGTCAGGAAGGATTCCAGTGAGAAAAAGCTCATCACAGATGCCCTGA
ATAAGAACCAGTTTCTGAAGAGATTGGATCCCCAGCAGATCAAGGACATGGTGGAATGCATGTACGGGAG
AAACTATCAACAGGGCAGCTACGTCATTAAGCAGGGGGAACCAGGCAACCATATCTTTGTGCTGGCCGAG
GGTAGACTTGAGGTGTTCCAGGGAGAGAAGTTGCTATCATCCATCCCAATGTGGACCACGTTTGGGGAAC
TGGCTATTTTGTACAACTGTACTCGGACTGCCTCAGTGAAAGCGATTACCAATGTTAAAACTTGGGCCCT
CGATAGAGAGGTTTTCCAGAACATTATGAGGAGAACAGCCCAAGCCCGGGATGAGGAGTACAGAAACTTC
CTCAGAAGTGTCTCCTTGTTGAAGAATTTACCTGAAGACAAACTAACCAAGATCATTGACTGCTTGGAAG
TGGAATACTATGACAAAGGAGATTACATCATCAGAGAAGGAGAAGAAGGGAGCACCTTTTTCATTTTGGC
AAAAGGCAAGGTGAAAGTCACACAGAGCACAGAGGGCCATGATCAGCCACAGTTGATCAAGACACTGCAG
AAAGGAGAGTACTTTGGAGAAAAGGCTCTGATCAGTGATGACGTCAGATCAGCCAATATAATTGCGGAAG
AAAATGATGTCGCATGCCTCGTGATAGATCGAGAAACATTCAACCAAACTGTCGGGACATTTGATGAACT
CCAAAAATATCTTGAGGGGTATGTGGCCACCCTGAACCGTGACGATGAAAAAAGACACGCAAAGCGCTCC
ATGTCTAGCTGGAAGCTGTCCAAAGCCCTTTCTCTGGAGATGATTCAGCTGAAAGAGAAGGTGGCCCGAT
TCTCCTCAACCTCCCCATTCCAGAACCTCGAGATTATCGCAACACTGGGCGTTGGTGGGTTCGGAAGAGT
GGAGCTTGTTAAAGTGAAAAATGAGAATGTTGCTTTTGCCATGAAGTGTATAAGGAAGAAGCACATTGTT
GATACAAAGCAGCAAGAACATGTCTACTCAGAAAAGAGGATCCTGGAAGAGCTGTGTTCTCCCTTCATCG
TGAAGTTGTATCGTACTTTCAAGGACAATAAATATGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGA
GCTGTGGAGTATACTGAGGGACAGAGGCAGCTTCGATGAACCCACCTCCAAATTCTGTGTCGCTTGTGTG
ACAGAAGCATTTGATTATCTGCACCGACTAGGCATCATCTACAGAGACCTGAAGCCAGAGAACTTAATTC
TGGATGCCGATGGCTACCTTAAGTTGGTTGACTTTGGATTCGCTAAGAAGATTGGCTCTGGACAGAAAAC
GTGGACATTCTGTGGGACTCCAGAGTATGTGGCCCCCGAGGTCATTCTTAACAAAGGACATGACTTCAGT
GTGGATTTCTGGTCCCTGGGGATTCTGGTCTATGAGCTCCTAACGGGCAACCCTCCCTTTTCTGGGATAG
ACCAAATGATGACCTACAATTTGATTCTCAAGGGAATCGAGAAAATGGATTTCCCCAGAAAGATAACAAG
GCGCCCTGAGGATTTGATCCGGAGGCTTTGCAGGCAAAACCCAACAGAAAGGCTGGGAAATCTGAAGAAC
GGGATCAACGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAAGGGCTGAAAGCACGGAGCC
TTCCGTCACCTTTACGGAGAGAGCTCAGTGGCCCCATAGATCACAGCTACTTTGACAAGTATCCTCCTGA
AAAGGGGGTGCCTCCGGATGAAATGTCAGGCTGGGATAAAGACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225140 protein sequence
Red=Cloning site Green=Tags(s)

MGNGSVKPKHAKHPDGHSGNLSNEALRSKVLELERELRRKDAELQEREYHLKELREQLAKQTVAIAELTE
ELQSKCIQLNKLQDVIHVQGGSPLQASPDKVPLDVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPP
EFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYVIKQGEPGNHIFVLAE
GRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEEYRNF
LRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQ
KGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFDELQKYLEGYVATLNRDDEKRHAKRS
MSSWKLSKALSLEMIQLKEKVARFSSTSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIV
DTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACV
TEAFDYLHRLGIIYRDLKPENLILDADGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFS
VDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKN
GINDIKKHRWLNGFNWEGLKARSLPSPLRRELSGPIDHSYFDKYPPEKGVPPDEMSGWDKDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008926
ORF Size 2286 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008926.4, NP_032952.3
RefSeq Size 4696 bp
RefSeq ORF 2289 bp
Locus ID 19092
Cytogenetics 5 48.35 cM
MW 87.3 kDa
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"NM_008926" in other vectors (4)
SKU Description Size Price
MC221428 Prkg2 (untagged) - Mouse protein kinase, cGMP-dependent, type II (Prkg2), (10ug) 10 ug
$768.00
MG225140 Prkg2 (tGFP-tagged) - Mouse protein kinase cGMP-dependent type II (Prkg2), (10ug) 10 ug
$899.00
MR225140L3 Lenti ORF clone of Prkg2 (Myc-DDK-tagged) - Mouse protein kinase, cGMP-dependent, type II (Prkg2) 10 ug
$999.00
MR225140L4 Lenti ORF clone of Prkg2 (mGFP-tagged) - Mouse protein kinase, cGMP-dependent, type II (Prkg2) 10 ug
$999.00

Plasmid Map for MR225140

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR225140
Circular map for MR225140

file-alt Citations

Filamin-ETV4/5 acts as mechanosensor-mechanotransducer axis that drives cell competition-mediated elimination of transformed cells
Wen, Sai, Masutani et al
Nat Commun (2026)
Cited by 1
AI Summary: OriGene contributed the pCMV-PRKG2-Myc plasmid (#MR225140), which was used in the study to investigate the role of PRKG2 in the mechanotransduction pathway driving the extrusion of transformed cells during cell competition.
Snippet: directed mutagenesis using specific primer sets. pCMV -PRKG2 -Myc (#MR225140) was from OriGene
Showing 1-1 of 1 papers.
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