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pCMV6-AC-3HA Mammalian Expression Vector

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SKU
PS100067
pCMV6-AC-3HA, mammalian vector with C-terminal 3XHA tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag 3 HA
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal 3XHA tag.
  • The string of 3 HA tag can enhance the binding affinity to anti-HA antibodies and therefore facilitate detection and purification of the tagged protein.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal 3XHA-tagging vector, PS100066.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

FRMD8 targets both CDK4 activation and RB degradation to suppress colon cancer growth
Yu, Wu, Sun et al
Cell Rep (2023) 42 (8), 112886 DOI: 10.1016/j.celrep.2023.112886
Cited by 15
AI Summary: OriGene’s pCMV6-AC-3HA plasmid (Cat#PS100067) was used in this study to facilitate the expression of tagged proteins, enabling the investigation of FRMD8’s molecular interactions and functional effects in colorectal cancer cells.
Snippet: Cyclin-dependent kinase 4 (CDK4) and retinoblastoma protein (RB) are both important cell-cycle regulators that function in different scenarios. Here, we report that FERM Save;Recombinant DNA p3×Flag-CMV-10 Solarbio Cat#VT000129 pCMV6-AC-3HA Origene Cat#PS100067 pGEX-4T-1 Solarbio Cat#VT000001
Oxidative stress-CBP axis modulates MOB1 acetylation and activates the Hippo signaling pathway
Jin, Zhang, Li et al
Nucleic Acids Res (2022) 50 (7), 3817-3834 DOI: 10.1093/nar/gkac189
Cited by 103
AI Summary: In this study, OriGene’s pCMV6-AC-3HA vector was used to construct HA-tagged MOB1 expression plasmids, enabling the investigation of MOB1 acetylation and its role in Hippo pathway activation.
Snippet: ces, Nankai University, Tianjin, China). FLAG-MOB1 and HA-MOB1 were constructed by PCR-amplified human full-length MOB1 cDNA inserting into p3 × FLAG-CMV-10 vector (Sigma) and pCMV6-AC-3HA vector (OriGene). The FLAG-MOB1 and HA-MOB1 mutants were constructed by using Muta-direct Kit (Sbsgene, SDM-15). The sequences of full-length (1–216), the N-terminal (1–110) and C-terminal (111–216)
FigureFigureFigure
Investigating the Redox Sensitivity of MITF Splice Variants
Berryman
Thesis (2020)
Snippet: A pCMV6-AC-3HA plasmid (Origene, PS100067) encoding a triple hemagglutinin (HA) carboxyl tag was utilized as the transfection vector. A BsiWI restriction site was substituted into the pCMV-Tag2B MITF variant plasmids using overlap extension PCR
Showing 1-3 of 3 papers.
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