ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a N-terminal 3XHA tag.
The string of 3 HA tag can enhance the binding affinity to anti-HA antibodies and therefore facilitate detection and purification of the tagged protein.
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal 3XHA-tagging vector, PS100067.
V2_F Primer can be used to sequence the target sequence after cloning.
XL39_R Primer can be used to sequence the target sequence after cloning.
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