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pCMV6-AN-3HA Mammalian Expression Vector

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SKU
PS100066
pCMV6-AN-3HA, mammalian vector with N-terminal 3XHA tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag 3 HA
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a N-terminal 3XHA tag.
  • The string of 3 HA tag can enhance the binding affinity to anti-HA antibodies and therefore facilitate detection and purification of the tagged protein.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal 3XHA-tagging vector, PS100067.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme
Li, Ivica, Dong et al
Nat Commun (2020) 11 (1), 4837 DOI: 10.1038/s41467-020-18607-1
Cited by 54
AI Summary: OriGene’s murine MFSD7C plasmid (Catalog No. MR208748) was used to generate MFSD7C-FLAG-Myc constructs for immunoprecipitation-mass spectrometry experiments, enabling the study of MFSD7C’s role in switching mitochondrial ATP synthesis to thermogenesis.
Snippet: … The MFSD7C-FLAG-Myc fragment was amplified from the murine MFSD7C plasmid (Origene Catalog No. MR208748) using the primers MFSD7C-SgfI-F and AC-Myc-DDK-MluI-KpnI-R. …MR208748) using the primers MFSD7C-SgfI-F and AC-Myc-DDK-MluI-KpnI-R. The fragment was digested with SgfI and then with KpnI. …
The angiotensin II type 2 receptors protect renal tubule mitochondria in early stages of diabetes mellitus
Micakovic, Papagiannarou, Clark et al
Kidney Int. (2018)
AI Summary: OriGene’s pCMV-AN-3HA vector was used to clone and overexpress the mouse AT2R in renal cells, enabling the study of its protective effects on kidney mitochondria in early diabetic nephropathy.
Snippet: The coding region of the mouse AT2R was amplified from the CMV-AT2R construct12 and then cloned in frame directly after the 3xHA sequence between the Sgfl1 and the Mlu1 site of the pCMV-AN-3HA (OriGene Technology GmbH, Herford, Germany). Methods are described in more detail in the Supplementary Materials and Methods
Showing 1-2 of 2 papers.
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