pCMV6-AN-mYFP Mammalian Expression Vector

SKU
PS100050
pCMV6-AN-mYFP, mammalian vector with N-terminal mYFP, 10ug
  $670.00
In Stock*
Specifications
Specifications
Product Data
N-Tag mYFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mYFP (monomeric) tag.
  • mYFP is a monomeric yellow fluorescent protein. mYFP possesses single excitation maximum at 508 nm, and emission maximum at 524 nm. mYFP is more pH stable than EYFP.
    mYFP is mainly intended for protein labeling in protein localization and interaction studies. It can also be used for cell and organelle labeling and for tracking the promoter activity, although TurboYFP is preferable for such applications because it mature faster and give brighter fluorescent signal.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal mYFP-tagging vector, PS100042.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Quantitative Micro-Spectroscopic Imaging Reveals Viral and Cellular RNA Helicase Interactions in Live Cells
Corby, Stoneman, Biener et al
J. Biol. Chem. (2017)
AI Summary: In this study, the researchers used the OriGene product pCMV3-RC204837 (LGP2/DHX58 cDNA) as a template to generate expression constructs for investigating interactions between viral and cellular RNA helicases in live cells.
Snippet: Oligonucleotides were purchased from Integrated DNA Technologies (IDT, Coralville, IA) pCMV-YFP-LGP2, pCMV-CMV-LGP2— LGP2 (DHX58) cDNA was amplified using PCR from pCMV3-RC204837 (OriGene, Inc.) primers which contain HindIII and XhoI restriction ... All 2 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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