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pCMV6-AC-mYFP Mammalian Expression Vector

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SKU
PS100042
pCMV6-AC-mYFP, mammalian vector with C-terminal mYFP, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag mYFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mYFP (monomeric) tag.
  • mYFP is a monomeric yellow fluorescent protein. mYFP possesses single excitation maximum at 508 nm, and emission maximum at 524 nm. mYFP is more pH stable than EYFP.
    mYFP is mainly intended for protein labeling in protein localization and interaction studies. It can also be used for cell and organelle labeling and for tracking the promoter activity, although TurboYFP is preferable for such applications because it mature faster and give brighter fluorescent signal.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mYFP-tagging vector, PS100050.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mYFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

The LIMP-2/SCARB2 binding motif on acid β-glucosidase: basic and applied implications for Gaucher disease and associated neurodegenerative diseases
Liou, Haffey, Greis et al
J. Biol. Chem. (2014)
AI Summary: OriGene's pCMV-AC-GFP/YFP/cMyc expression vectors were used in this study to facilitate the heterologous expression of GCase constructs, enabling the investigation of GCase-LIMP-2 binding motifs relevant to Gaucher disease.
Snippet: MES SDS running buffer, DMEM, pBluescript vector, Dynabeads protein G Immuno- precipitation Kits, BS3 chemical cross linker (Invitrogen, Carlsbad, CA);BCA Protein Assay Reagent (Pierce, Rockford, IL);pCMV- AC-GFP/YFP/cMyc expression vectors (Origene, Rockville ... Cite SaveSaving
Selective Recapitulation of Conserved and Non-Conserved Regions of Putative NOXA1 Activation Domain Confers Isoform-Specific Inhibition of Nox1 Oxidase, Attenuation of Endothelial Cell Nox and Migration
Ranayhossaini, Rodriguez, Sahoo et al
J. Biol. Chem. (2013)
AI Summary: OriGene provided custom subclones of Nox1-YFP and NOXA1-CFP plasmids, which were used in FRET experiments to study the interaction between Nox1 and NOXA1 in this paper.
Snippet: Plasmids encoding full- length human cDNAs for Nox1-YFP and NoxA1- CFP were custom subclones purchased from OriGene (Rockville, MD). Nox1-YFP was subcloned into the pCMV6-AC-mYFP plasmid and NOXA1-CFP was subcloned into the pCMV6- AC-mCFP ... Cite SaveSaving
Showing 1-2 of 2 papers.
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