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pCMV6-AC-YFP Mammalian Expression Vector

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SKU
PS100036
pCMV6-AC-YFP, mammalian vector with C-terminal tYFP tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag TurboYFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal tYFP tag.
  • TurboYFP (excitation/ emission max = 525/ 538nm) is an enhanced variant of the yellow fluorescent protein PhiYFP. TurboYFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboYFP
  • Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
  • If the N-terminal tagging interferes with the protein’s function, you can choose the N-terminal tYFP-tagging vector, PS100035
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • YFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

MAP4K3/GLK promotes lung cancer metastasis by phosphorylating and activating IQGAP1
Chuang, Chang, Teng et al
Cancer Res. (2019)
AI Summary: OriGene products, specifically the pCMV6-AN-3DDK, pCMV6-AC-HA, pCMV6-AC-mCFP, and pCMV6-AN-mGFP expression vectors, were used to generate tagged GLK constructs for studying the interaction and functional effects of GLK and IQGAP1 in lung cancer metastasis.
Snippet: the Flag-tagged clone into the vector pCMV6-AN-3DDK or pCMV6-AC-HA (OriGene,) insert from the HA-tagged GLK plasmid into the vector pCMV6-AC-mCFP and pCMV6-AN-mGFP (OriGene), respectively. The plasmids expressing YFP-fused
GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease
Chuang, Tsai, Hsueh et al
Sci Adv (2018) 4 (9), eaat5401 DOI: 10.1126/sciadv.aat5401
AI Summary: OriGene products, specifically the pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, and pCMV6-AC-Flag vectors, were used to construct plasmids for expressing tagged proteins (such as PKCθ and AhR) that enabled the study of protein interactions and signaling mechanisms underlying IL-17A induction in autoimmune disease.
Snippet: PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …
FigureFigureFigure
HGK/MAP4K4 deficiency induces TRAF2 stabilization and Th17 differentiation leading to insulin resistance
Chuang, Sheu, Lin et al
Nat Commun (2014) 5, 4602 DOI: 10.1038/ncomms5602
Cited by 88
AI Summary: In this study, OriGene’s HGK (MAP4K4) shRNA (TG502527) and TRAF2 cDNA (SC118226) were used to manipulate gene expression in T cells, enabling the authors to investigate the molecular mechanisms by which HGK deficiency leads to TRAF2 stabilization, Th17 differentiation, and insulin resistance.
Snippet: 46. Park, E. J. et al. Dietary and genetic obesity promote liver inflammation and tumorigenesis by enhancing IL-6 and TNF expression. Cell 140, 197–208 (2010)
Showing 1-3 of 3 papers.
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