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pCMV6-AN-YFP Mammalian Expression Vector

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SKU
PS100035
pCMV6-AN-YFP, mammalian vector with N-terminal tYFP tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag TurboYFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal tYFP tag.
  • TurboYFP (excitation/ emission max = 525/ 538nm) is an enhanced variant of the yellow fluorescent protein PhiYFP . TurboYFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboYFP
  • Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal tYFP-tagging vector, PS100036
  • YFP_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells
Zamponi, Zamponi, Coskun et al
Aging Cell (2018)
AI Summary: OriGene’s mitochondrial-targeted YFP plasmid was used in this study to visualize mitochondria, enabling assessment of mitochondrial structure and function in Down syndrome cells.
Snippet: for cyto- plasmic expression (cCAT). The mitochondrial‐targeted YFP plasmid was purchased from OriGene and the ORF subcloned into the same pLenti vector (mitoYFP). 4.3 | shRNA silencing vector The short hairpin RNA
Showing 1-1 of 1 papers.
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