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pCMV6-AC-RFP Mammalian Expression Vector

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SKU
PS100034
pCMV6-AC-RFP, mammalian vector with C-terminal tRFP tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag TurboRFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal tRFP tag
  • TurboRFP (excitation/ emission max = 553/ 574 nm) is a red (orange) fluorescent protein. TurboRFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboRFP
  • Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal tRFP-tagging vector, PS100033
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • RFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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Activity-regulated circSamm50 modulates mitochondrial dynamics and spine structural plasticity
Chanda, Bapat, Wingfield et al
Cell Rep (2026) 45 (6), 117378 DOI: 10.1016/j.celrep.2026.117378
AI Summary: In this study, OriGene products pCMV6-AC-GFP (PS100010) and pCMV6-AC-RFP (PS100034) were used as fluorescent expression vectors to facilitate visualization and analysis of gene expression and protein localization related to circSamm50’s role in mitochondrial dynamics and synaptic plasticity.
Snippet: pCMV6-AC-GFP Origene PS100010 pGL3 Basic Luciferase vector Promega E1751 pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP Addgene 228359 Samm50-WT-pGL3 This paper N/A Samm50-MUT-pGL3 This paper N/A circSamm50-WT- pGL3 This paper N/A circSamm50-MUT-pGL3 This paper N/A RCaMP1.07 This paper N/A pCMV6-AC-RFP Origene PS100034
eIF2B localisation and its regulation during the integrated stress response is cell-type specific
Hanson, Ribeiro de Oliveira, Cross et al
iScience (2024)
Cited by 6
AI Summary: OriGene products, specifically the pCMV6-AC-tGFP plasmid vectors encoding EIF2B5 and EIF2S1, were used to generate and verify expression constructs for studying eIF2B localisation and regulation in this research.
Snippet: pCMV6-AC-tGFP plasmid vector encoding EIF2B5 and EIF2S1 was purchased from 697 OriGene (Rockville, Maryland, USA). The coding open reading frame of EIF2B5 from 698 the pCMV6-AC-tGFP vector was cloned into an empty pCMV6-AC-mGFP and empty 699 pCMV6-AC-mRFP vectors. The constructs were verified by sequencing
α-Synuclein antisense transcript SNCA-AS1 regulates synapses- and aging-related genes suggesting its implication in Parkinson's disease
Rey, Pandini, Messa et al
Aging Cell (2021) 20 (12), e13504 DOI: 10.1111/acel.13504
Cited by 31
AI Summary: OriGene products, specifically the pCMV6-AC-RFP construct for SNCA-AS1 and the alpha Synuclein SNCA NM_000345 Human Tagged ORF Clone (RG210606), were used to generate stable cell lines overexpressing SNCA-AS1 and SNCA, enabling the study of their roles in synaptic regulation and aging-related pathways in Parkinson’s disease.
Snippet: and 1% penicillin/streptomycin (Gibco) at 37°C in a 5% CO2 atmosphere. For SNCA‐AS1 stable transfection, a pCMV6‐AC‐RFP construct containing gentamicin‐disulfate G418 resistance was used (OriGene). For SNCA stable transfection, a custom pCMV6‐AC‐GFP construct containing G418 resistance was used (alpha Synuclein SNCA NM_000345 Human Tagged ORF Clone, RG210606, OriGene). The plasmids
Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis
Sønder, Häger, Heitmann et al
Sci Adv (2021) 7 (27) DOI: 10.1126/sciadv.abg1969
Cited by 82
AI Summary: In this study, OriGene products ANXA4 (OriGene, #RG203229) and the pCMV6-AC-tRFP vector (OriGene, #PS100034) were used to generate the ANXA4-tRFP construct, enabling visualization and analysis of protein dynamics during plasma membrane restructuring after damage.
Snippet: ANXA4-tRFP had been generated by cloning ANXA4 (Origene, #RG203229) into the pCMV6-AC-tRFP vector (Origene, #PS100034) (2). The eGFP-LC3 was a gift from T. Yoshimori (49). …
Simultaneous membrane binding of Annexin A4 and A5 suppresses 2D lattice formation while maintaining curvature induction
Mularski, Sønder, Heitmann et al
J Colloid Interface Sci (2021) 600, 854-864 DOI: 10.1016/j.jcis.2021.05.067
Cited by 14
AI Summary: OriGene products, specifically the turbo-GFP C-terminal tagged human ANXA4 (RG203229) and ANXA5 (RG205619) expression plasmids, were used to transiently express labeled annexins in MCF7 cells, enabling the study of their membrane localization and cooperative behavior during plasma membrane repair.
Snippet: …Expression plasmids with turbo-GFP C-terminal tag containing human ANXA4 or ANXA5 were purchased from OriGene Technologies (OriGene RG203229 and RG205619). ANXA4-tRFP;- 4. … Expression plasmids with turbo-GFP C-terminal tag containing human ANXA4 or ANXA5 were purchased from OriGene Technologies (OriGene RG203229 and RG205619). ANXA4-tRFP
Pharmacological targeting of differential DNA repair, radio-sensitizes WRN-deficient cancer cells in vitro and in vivo
Gupta, Saha, Chattopadhyay et al
Biochem Pharmacol (2021) 186, 114450 DOI: 10.1016/j.bcp.2021.114450
Cited by 31
AI Summary: OriGene’s pCMV6-AC-RFP plasmid was used in this study for transfection experiments to investigate the molecular mechanisms underlying DNA repair and radiosensitization in WRN-deficient cancer cells.
Snippet: Addgene (#26477)] and pCMV6-AC-RFP plasmid [Origene Technologies (Rockville, MD)] expression plasmid using lipofectamine 2000 for 48 h and kept under dark
Showing 1-6 of 17 papers.
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