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pCMV6-AN-RFP Mammalian Expression Vector

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SKU
PS100033
pCMV6-AN-RFP, mammalian vector with N-terminal tRFP tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
N-Tag TurboRFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal tRFP tag.
  • TurboRFP (excitation/ emission max = 553/ 574 nm) is a red (orange) fluorescent protein. TurboRFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboRFP
  • Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal tRFP-tagging vector, PS100034
  • RFP_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Genome-wide dissection reveals diverse pathogenic roles of bacterial Tc toxins
Song, Chen, Zhou et al
PLoS Pathog (2021) 17 (2), e1009102 DOI: 10.1371/journal.ppat.1009102
Cited by 34
AI Summary: In this study, OriGene’s pCMV6 vectors (PS100019 for GFP and PS100033 for RFP) were used as sources for cloning the GFP and RFP coding sequences, enabling fluorescent tagging in the analysis of bacterial Tc toxins.
Snippet: entomophaga and PTP-HVR of TccC1 (AKB86704.1) of Yersinia pestis KIM10+ were synthesized by Ruibio BioTech. The coding sequences of GFP and RFP were cloned from pCMV6 vectors (PS100019 and PS100033, Origene). TcdA1 of Photorhabdus luminescens W14 was cloned into RSFDuet-1 (Novagen). All TcB-TcC fusion proteins were constructed incorporating a 5-aa linker region encoding Arg-Gly-Ser-Arg-Pro, and c
FigureFigureFigure
Genetic contribution of retinoid-related genes to neural tube defects
Li, Zhang, Chen et al
Hum Mutat (2018) 39 (4), 550-562 DOI: 10.1002/humu.23397
Cited by 37
AI Summary: In this study, OriGene’s CYP26B1 (RC217445) and ALDH1A2 (RC203307) expression plasmids were used to functionally analyze the impact of neural tube defect-associated gene variants on retinoic acid metabolism.
FigureFigure
Ceramide Synthase 6 Is a Novel Target of Methotrexate Mediating Its Antiproliferative Effect in a p53-Dependent Manner
Fekry, Esmaeilniakooshkghazi, Krupenko et al
PLoS ONE (2016) 11 (1), e0146618 DOI: 10.1371/journal.pone.0146618
Cited by 52
AI Summary: OriGene products pCMV6-AC-RFP/CALR and pCMV6-AN-RFP/MAP1LC3B expression vectors were used in this study to investigate protein localization and cellular processes related to CerS6 function and methotrexate response in cancer cells.
Snippet: pEGFP vector (Addgene, Cambridge, MA) downstream of GFP. pCMV6-AC-RFP/CALR and pCMV6-AN-RFP/MAP1LC3B vectors were purchased from OriGene (Rockville, MD). siRNA. The siRNA knockdown of CerS6 or CerS4 ... Cite SaveSaving
FigureFigureFigure
Rab14 regulates the maturation of macrophage phagosomes containing the fungal pathogen Candida albicans and the outcome of the host-pathogen interaction
Okai, Lyall, Gow et al
Infect. Immun. (2015)
AI Summary: OriGene products, specifically murine Rab5 and Rab7 cDNA plasmids, were used as sources for gene amplification and cloning to study the roles of these Rab GTPases in macrophage phagosome maturation during Candida albicans infection.
Snippet: Rab7 were constructed as previously described (18): cDNA of murine Rab5 was 147 amplified from plasmid (OriGene, Rockville, MD, USA) using a proof-reading 148 ... USA). Murine Rab7 cDNA was digested from plasmid (Origene) with SgfI and MluI 153 ... Cite SaveSaving
Distribution of insulin mRNA transcripts within the human body
Bell, Reddy, Sun et al
Biochem. Biophys. Res. Commun. (2014) 451 (3), 425-30 DOI: 10.1016/j.bbrc.2014.07.140
Cited by 4
AI Summary: OriGene’s TissueScan Human Normal (Cat# HMRT102) and TissueScan Human Brain (Cat# HBRT101) cDNA panels were used to screen for insulin mRNA transcripts across diverse human tissues, enabling the authors to identify rare insulin mRNA-expressing cells outside the pancreas.
Snippet: 2.1. Tissue cDNAs. Panels of cDNAs from 48 different types of human tissues (TissueScan Human Normal, Cat# HMRT102), and from 24 regions of the human brain (TissueScan Human Brain, Cat# HBRT101) were obtained from OriGene (Rockville, MD, USA). ... Cite SaveSaving
Targeting of aquaporin 4 into lipid rafts and its biological significance
Asakura, Ueda, Shima et al
Brain Res. (2014)
Cited by 11
AI Summary: OriGene's pCMV6-AC-RFP vector was used to clone the AQP4 M1 isoform, enabling the creation of fluorescently tagged cell lines that were essential for studying the membrane targeting of aquaporin 4 in this research.
Snippet: The AQP4 M1 PCR product was first inserted into the pCMV6-AC- RFP vector (Origene, Rockville, MD), and the AQP4 M23 PCR product was inserted into the pEF1α-AcGFP- N1 vector (Clontech Laboratories Inc., Mountainview, CA). ... Cite SaveSaving
Showing 1-6 of 7 papers.
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