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pCMV6-AN-His-DDK Mammalian Expression Vector

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SKU
PS100018
pCMV6-AN-His-DDK, mammalian vector with N-terminal His-DDK tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag His-DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed as a tagged protein with the N-terminal His-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene expression using anti-DDK or anti-His antibodies or a nickel affinity columns
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal His-DDK-tagging vector, PS100009
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Transcriptional repression of the ectodomain sheddase ADAM10 by TBX2 and potential implication for Alzheimer's disease
Reinhardt, Schuck, Stoye et al
Cell. Mol. Life Sci. (2019)
AI Summary: OriGene products contributed to this study by providing expression vectors for HDAC1 (pCMV-Entry_Myc_DDK_HDAC1), TBX2 (pCMV6_TBX2), TBX5 (pCMV6_TBX5), and TBX21 (pCMV6_TBX21), which were used to investigate the transcriptional repression of ADAM10 by TBX2.
Snippet: Expression vectors for HDAC1 (pCMV-Entry_Myc_DDK_HDAC1), TBX2 (pCMV6_TBX2), TBX5 (pCMV6_TBX5), TBX21 (pCMV6_TBX21) and respective empty vector were obtained from Origene (Rockville, MD, USA)
Gene-gene interaction and RNA splicing profiles of MAP2K4 gene in rheumatoid arthritis
Shchetynsky, Protsyuk, Ronninger et al
Clin. Immunol. (2015) 158 (1), 19-28 DOI: 10.1016/j.clim.2015.02.011
Cited by 20
AI Summary: OriGene’s PrecisionShuttle mammalian vector (PS100018) was used to generate transfection plasmids expressing canonical and variant MAP2K4 cDNAs, enabling functional studies of MAP2K4 splice forms in the investigation of their role in rheumatoid arthritis.
Snippet: Transfection plasmids E5 and noE5 were based on cDNA sequences of the canonical MAP2K4 and the putative variant (lacking exon 5) respectively — inserted into a PrecisionShuttle mammalian vector with N-terminal His-DDK tag (PS100018, OriGene ... Cite SaveSaving
Showing 1-2 of 2 papers.
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