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pCMV6-AC-DDK-His Mammalian Expression Vector

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SKU
PS100009
pCMV6-AC-DDK-His, mammalian vector with C-terminal DDK-His tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag DDK-His
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed as a tagged protein with the C-terminal DDK-His tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene expression using anti-DDK or anti-His antibodies or a nickel affinity columns
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal DDK-His-tagging vector, PS100018.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Engineering polysialic acid on Schwann cells using polysialyltransferase gene transfer or purified enzyme exposure for spinal cord injury transplantation
Pearse, Rao, Morales et al
Neurosci Lett (2021) 748, 135690 DOI: 10.1016/j.neulet.2021.135690
Cited by 5
AI Summary: In this study, OriGene’s TrueORF cDNA clone for mouse ST8SIA4 (Cat# MR209993) was used to generate lentiviral vectors for polysialyltransferase gene transfer, enabling the researchers to induce polysialic acid expression on Schwann cells and assess its effects on migration and axon growth after spinal cord injury transplantation.
Quantitative glycoproteomics reveals new classes of STT3A- and STT3B-dependent N-glycosylation sites
Cherepanova, Venev, Leszyk et al
J. Cell Biol. (2019) 218 (8), 2782-2796 DOI: 10.1083/jcb.201904004
AI Summary: OriGene's pCMV6-AC-DDK-His vector was used to clone and express GRP94 and KDEL2 coding sequences, enabling the study of glycosylation patterns in the analyzed proteins.
Snippet: A full-length GRP94-verified clone was purchased from the Human Clone Collection at University of Massachusetts Medical School (ID 6165138). A KDEL2 clone was purchased from Transomics (ID 5266665). The coding sequences were amplified by PCR and cloned into the pCMV6-AC-DDK-His vector (Origene)
DC2 and KCP2 mediate the interaction between the oligosaccharyltransferase and the ER translocon
Shrimal, Cherepanova, Gilmore
J. Cell Biol. (2017)
AI Summary: OriGene's pCMV6-AC-DDK-His vector was used for cloning amplification products, facilitating the expression and analysis of proteins involved in the study of interactions between the oligosaccharyltransferase and the ER translocon.
Snippet: The amplification products were then cloned into a pCMV6-AC-DDK-His vector (OriGene) with a stop codon inserted upstream of the DDK-His coding sequence
Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab
Ramakrishna, Sundarapandiyan, Zhao et al
J Immunother Cancer (2015) 3, 37 DOI: 10.1186/s40425-015-0080-2
Cited by 62
AI Summary: In this study, OriGene's Human CD27 (TNFRSF7) cDNA clone (Cat# SC119755) was used to characterize the molecular and functional effects of varlilumab-mediated CD27 costimulation in human T cells.
FigureFigureFigure
Regulation of NEIL1 protein abundance by RAD9 is important for efficient base excision repair
Panigrahi, Hopkins, Lieberman
Nucleic Acids Res. (2015)
Cited by 16
AI Summary: OriGene contributed to this study by providing the mouse Neil1 ORF clone (#MC211355), which was used to investigate the role of NEIL1 in base excision repair regulated by RAD9.
Snippet: All cell lines were cultured at 37°C, 5% CO 2. Plasmid construction and generation of cell lines. Mouse Neil1 and human NEIL1 ORF clones were purchased from Origene (#MC211355) and Genecopoeia (#A3833), respectively. ... Cite SaveSaving
Reduced expression of the oligosaccharyltransferase exacerbates protein hypoglycosylation in cells lacking the fully assembled oligosaccharide donor
Shrimal, Gilmore
Glycobiology (2015)
Cited by 16
AI Summary: In this study, OriGene’s pCMV6-AC-DDK-His vector was used to express human prosaposin and progranulin, enabling the authors to investigate how altered glycosylation affects these proteins in cells with defects in the oligosaccharide donor and OST subunit expression.
Snippet: Human prosaposin and progranulin were amplified from cDNA clones (Thermo Scientific, Pittsburg, PA, USA) and inserted into the pCMV6-AC-DDK-His vector (OriGene, ... (Origene) and inserted into pCDNA-V5H6. A common polymorphism that can cause a mild ... Cite SaveSaving
Showing 1-6 of 12 papers.
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