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pCMV6-AN-His-Myc Mammalian Expression Vector

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SKU
PS100015
pCMV6-AN-His-Myc, mammalian vector with N-terminal Myc-His tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag His-Myc
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the N-terminal Myc-His tags.
  • Such clones are the best for detection and purification of the transgene expression using anti-Myc or anti His antibodies or a nickel affinity column
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Myc-His-tagging vector, PS100006
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

N-glycosylation and expression in human tissues of the orphan GPR61 receptor
Kozielewicz, Alomar, Yusof et al
FEBS Open Bio (2017) 7 (12), 1982-1993 DOI: 10.1002/2211-5463.12339
Cited by 12
AI Summary: OriGene’s pCMV6-An-His-Myc vector was used to clone and express wild-type and mutant GPR61 proteins, enabling the study of their N-glycosylation and tissue-specific expression in this research.
Snippet: human tissues. Materials and methods Cell culture and cloning Wild-type GPR61 plasmid DNA (R&D Systems) was inserted into pCMV6-An-His-Myc vector (OriGene) using HindIII and XhoI restriction enzymes. The N12S mutant
Showing 1-1 of 1 papers.
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