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pCMV6-AC-Myc-His Mammalian Expression Vector

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SKU
PS100006
pCMV6-AC-Myc-His, mammalian vector with C-terminal Myc-His tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-His
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal Myc-His-tag.
  • Such clones are the best for detection and purification of the transgene expression using anti-Myc or anti His antibodies or a nickel affinity column
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Myc-His-tagging vector, PS100015.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
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EGCG Disrupts the LIN28B/Let-7 Interaction and Reduces Neuroblastoma Aggressiveness
Cocchi, Greco, Sidarovich et al
Int J Mol Sci (2024) 25 (9) DOI: 10.3390/ijms25094795
Cited by 5
AI Summary: OriGene’s pCMV6-AC-Myc-His mammalian expression vector (cat. PS100006) was used to clone and express full-length human LIN28B cDNA, enabling the study of LIN28B/let-7 interactions in neuroblastoma cells.
Snippet: The full-length human LIN28B cDNA sequence (NM_001004317.3) was amplified from retro-transcribed RNA of HEK293 cells and inserted into the pCMV6-AC-Myc-His mammalian expression vector (cat. PS100006, Origene Technologies, Rockville, MD, USA) by using the forward
FigureFigureFigure
Elafin reverses intestinal fibrosis by inhibiting cathepsin S-mediated protease-activated receptor 2
Xie, Fontenot, Estrada et al
Cell Mol Gastroenterol Hepatol (2022) 14 (4), 841-876 DOI: 10.1016/j.jcmgh.2022.06.011
Cited by 20
AI Summary: In this study, OriGene products—including Elafin overexpressing lentivirus, Mouse Zeb1 shRNA lentivirus, Mouse Zeb1 overexpressing lentivirus, and Mouse Ctss siRNA lentivirus—were used to manipulate gene expression in fibroblasts and mouse models, enabling the investigation of elafin’s anti-fibrotic mechanisms in intestinal fibrosis.
Snippet: 797 hydrochloride Sigma SML1939 98% 57328 Control lentivirus Elafin overexpressing Lentivirus Mouse Zeb1 shRNA lentivirus Mouse Zeb1 overexpressing lentivirus Mouse Ctss siRNA lentivirus OriGene OriGene OriGene OriGene ABMgood PS1000064V RC203136L1V TL513177V MR223095L2V 171210940296 N/A N/A N/A N/A N/A 133EE26 SR156301 SR168040 SR180321 V21H18I Mouse Ctss overexpressing lent
Ursodeoxycholic acid reduces antitumor immunosuppression by inducing CHIP-mediated TGF-β degradation
Shen, Lu, Song et al
Nat Commun (2022) 13 (1), 3419 DOI: 10.1038/s41467-022-31141-6
Cited by 112
AI Summary: OriGene products, specifically the plasmids PCMV6-TGF-β1-Myc-Flag (MR227339), PCMV6-CHIP-Myc-Flag (MR204258), PCMV6-PRKACA-Myc-Flag (MR205322), and PCMV6-p62-Myc-Flag (MR226105), were used in this study to investigate the molecular mechanisms by which ursodeoxycholic acid promotes TGF-β degradation and enhances antitumor immunity.
Snippet: … The PCMV6-TGF-β1-Myc-Flag (MR227339), PCMV6-CHIP-Myc-Flag (MR204258), PCMV6-PRKACA-Myc-Flag (MR205322) and PCMV6-p62-Myc-Flag (MR226105) plasmids were purchased from Origene Technologies (Rockwell, Maryland, USA). The PCMV6-TGF-β1-Flag, TGF-β1 mutant, PCMV6-CHIP-Flag, PCMV6-CHIP-His, PCMV6-PRKACA-Myc, pMX-PRKACA and PCMV6-p62_UBAΔ-Myc plasmids were constructed;, PCMV6-CHIP-Myc-Flag (MR204258), PCMV6-PRKACA-Myc-Flag (MR205322) and PCMV6-p62-Myc-Flag (MR226105) plasmids were purchased from Origene Technologies (Rockwell, Maryland, USA)
ELK3 Controls Gastric Cancer Cell Migration and Invasion by Regulating ECM Remodeling-Related Genes
Lee, Cho, Park et al
Int J Mol Sci (2022) 23 (7) DOI: 10.3390/ijms23073709
Cited by 50
AI Summary: OriGene contributed to this study by providing the pLenti-cMyc-DDK vector (PS100064), which was used to generate ELK3-overexpressing constructs for investigating the role of ELK3 in gastric cancer cell migration and invasion.
Snippet: i-cMyc-DDK-ELK3 using Lipofectamine 2000 reagent. pLenti-cMyc-DDK-ELK3 was generated from cDNA by PCR. The protein coding region of ELK3 gene was ligated into the pLenti-cMyc-DDK vector (PS1000064;OriGene Technologies, Inc., Rockville, MD, USA). The transfected cells were incubated in 37 °C for 48 h to 72 h. The efficacy of transient transfection was approximately 30% in SNU638 cells. To gener
FigureFigureFigure
Upregulation of CD36, a Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis by Increasing MMP28 and Decreasing E-Cadherin Expression
Drury, Rychahou, Kelson et al
Cancers (Basel) (2022) 14 (1) DOI: 10.3390/cancers14010252
Cited by 64
AI Summary: OriGene products pLenti-C-Myc-DDK-P2A-Puro-CD36 overexpression (RC221976L1V) and pLenti-C-Myc-DDK-P2A-Puro-Empty-Vector (PS1000064) were used to generate colorectal cancer cell lines with altered CD36 expression, enabling the study of CD36's role in cancer metastasis in this paper.
Snippet: prior to performing in vitro and in vivo experiments. Overexpression cell lines were established by transfecting HCT116 and HT29 LuM0 cells with either pLenti-C-Myc-DDK-P2A-Puro-CD36 overexpression (OriGene #RC221976L1V) or pLenti-C-Myc-DDK-P2A-Puro-Empty-Vector (OriGene #PS1000064) lentiviral transduction particles. MMP28 knockdown cell lines were generated by transfecting CRC cells with scrambl
FigureFigureFigure
The role of autophagy in age-related macular degeneration
Kivinen
Acta Ophthalmol (2018) 96 Suppl A110, 1-50 DOI: 10.1111/aos.13753
AI Summary: OriGene's pCMV6-AC-Myc-His PrecisionShuttle vector (PS100006) was used to clone and express the ELAVL1/HuR cDNA for functional studies in this paper.
Snippet: ELAVL1/HuR cDNA (NM 001419) was obtained and amplified from MCF7‐cells retro‐transcribed RNA and inserted into the pCMV6‐AC‐Myc‐His PrecisionShuttle vector (PS100006;Origene Technologies) by using the forward (5′‐ GCC GCGATCGC CATGTCTAATGGTTATGA‐3′) and reverse (5′‐ CGT ACGCGT TTTGTGGGACTTGTTGG‐3′) primers containing the SgfI and the MluI restriction sites (in italics), respectively. …
Showing 1-6 of 11 papers.
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