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pCMV6-AN-HA Mammalian Expression Vector

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SKU
PS100013
pCMV6-AN-HA, mammalian vector with N-terminal HA tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag HA
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a N-terminal HA-tag.
  • Such clones are the best for detection and purification of the transgene expression using an anti-HA antibody.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal HA-tagging vector, PS100004
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Identification of B56α, B56γ and BAP1 as PRR14L binding partners
Chase, Carreño-Tarragona, Lin et al
bioRxiv (2023)
Cited by 1
Snippet: Both were inserted into pCMV6-AN-HA (Origene, #PS100013) and the correct sequence confirmed by sequencing. The inserts correspond to references sequences
Novel biomimetic mesoporous silica nanoparticle system possessing targetability and immune synergy facilitates effective solid tumor immuno-chemotherapy
Zhu, Liu, Yi et al
Biomater Adv (2023) 144, 213229 DOI: 10.1016/j.bioadv.2022.213229
Cited by 21
AI Summary: OriGene’s pCMV6-AN-DDK (PS100014) mammalian expression vector was used to clone the chimeric anti-HER2 scFv-LFA3 cDNA, enabling the production of targeting molecules critical for the biomimetic nanoparticle system developed in this study.
Snippet: [37]. Chimeric anti-Her2 scFv-LFA3 cDNA was cloned into pCMV6-AN-DDK (PS100014, Origene, USA), a mammalian expression vector with an N-terminal DDK tag. T
Novel biomimetic mesoporous silica nanoparticles system possessed targetability and immune synergy to promote solid tumor immuno- chemotherapy efficacy
Zhang, Zhu, Yi et al
Research Square (2022)
Snippet: GPI-CD80 cDNA was constructed by fusing cDNA encoding the extracellular domain of CD80 and the GPI-anchor signal sequence of LFA-3 [29;30]. The chimeric CD80-LFA3 cDNA was ligated into pCMV6-AN-HA (PS100013, Origene, USA), a mammalian expression vector with N-terminal HA tag. …
Solid tumor target-immunotherapy with novel biomimetic immuno- chemotherapy composite mesoporous silica nanoparticles coated by the CD80 modified cell membranes
Wo, Zhang, Zhu et al
Research Square (2022)
Snippet: GPI-CD80 cDNA was constructed by fusing cDNA encoding the extracellular domain of CD80 and the GPIanchor signal sequence of LFA-3 [28;29] . The chimeric CD80-LFA3 cDNA was ligated into pCMV6-AN-HA (PS100013, Origene, USA), a mammalian expression vector with N-terminal HA tag. For construction of GPI-anchored anti-Her2 scFv
Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer
Barger, Chee, Albahrani et al
Elife (2021) 10 DOI: 10.7554/eLife.55070
AI Summary: OriGene products, specifically the RHNO1 Myc-DDK-tagged cDNA (RC209091) and FOXM1 Myc-DDK-tagged cDNA (RC206307), were used in this study to overexpress RHNO1 and FOXM1 in cancer cell lines, enabling the authors to investigate the functional roles of these genes in oncogenic processes.
FigureFigureFigure
DNA Polymerase ι Interacts with Both the TRAF-like and UBL1-2 Domains of USP7
Ashton, Valles, Jaiswal et al
J Mol Biol (2020) 433 (2), 166733 DOI: 10.1016/j.jmb.2020.166733
Cited by 20
AI Summary: OriGene’s pCMV6-AN-HA plasmid was used in this study as the expression vector for ubiquitin, enabling the investigation of protein interactions and deubiquitination mechanisms involving DNA polymerase ι and USP7.
Snippet: … The coding sequence of ubiquitin was similarly synthesized and subcloned into the AsiSI and MluI restriction sites of pCMV6-AN-HA (Origene). Pol ι and USP7 truncation and point mutants were constructed by synthesizing new gene fragments (Genscript) and subcloning into the wild-type plasmids using restriction sites flanking or co;The coding sequence of ubiquitin was similarly synthesized and subcloned into the AsiSI and MluI restriction sites of pCMV6-AN-HA (Origene)
Showing 1-6 of 20 papers.
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