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pCMV6-AC-HA Mammalian Expression Vector

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SKU
PS100004
pCMV6-AC-HA, mammalian vector with C-terminal HA tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag HA
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal HA-tag.
  • Such clones are the best for detection and purification of the transgene expression using an anti-HA antibody.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal HA-tagging vector, PS100013.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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MICU1 regulates mitochondrial cristae structure and function independently of the mitochondrial Ca2+ uniporter channel
Tomar, Thomas, Garbincius et al
Sci Signal (2023) 16 (782), eabi8948 DOI: 10.1126/scisignal.abi8948
Cited by 64
AI Summary: In this study, OriGene’s MICU1-FLAG plasmid (#MR207652) and pCMV6-AC-HA vector (#PS100004) were used to generate tagged MICU1 constructs for investigating the role of MICU1 in mitochondrial structure and function.
Snippet: MICU1 is a calcium (Ca 2+ )-binding protein that regulates the mitochondrial Ca 2+ uniporter channel complex (mtCU) and mitochondrial Ca 2+ uptake. MICU1 knockout Save;The MICU1-HA plasmid was generated by cleaving the MICU1 fragment from the MICU1-FLAG plasmid (OriGene, #MR207652) using the Sgf I–Mlu I restriction sites and inserted into the same sites in the pCMV6-AC-HA vector (OriGene, #PS100004). Flag-tagged mouse MICU1 (NM_144822) mutants;EMREmut KKKKR101-105QQQQQ
Malat1 deficiency prevents neonatal heart regeneration by inducing cardiomyocyte binucleation
Aslan, Jaé, Manavski et al
JCI Insight (2023) 8 (5) DOI: 10.1172/jci.insight.162124
Cited by 15
AI Summary: OriGene Technologies contributed to this study by providing wild-type and mutant hnRNP U expression plasmids, both constructed using the pCMV6-AC-HA mammalian expression vector (OriGene, PS100004), which were used to investigate the role of hnRNP U in cardiomyocyte proliferation and binucleation.
Snippet: …Both plasmids were designed by OriGene Technologies using the pCMV6-AC-HA mammalian expression vector as backbone (OriGene, PS100004). … Cells were seeded 24 hours be;- Both plasmids were designed by OriGene Technologies using the pCMV6-AC-HA mammalian expression vector as backbone (OriGene, PS100004). …
Glucocorticoid Receptor β Isoform Predominates in the Human Dysplastic Brain Region and Is Modulated by Age, Sex, and Antiseizure Medication
Westcott, Chung, Ghosh et al
Int J Mol Sci (2022) 23 (9) DOI: 10.3390/ijms23094940
Cited by 7
AI Summary: OriGene products contributed to this study by providing the custom HA-tagged GRβ plasmid (cat. RC220377) cloned in the pCMV6-AC-HA vector (cat. PS100004), which was used to overexpress GRβ in brain endothelial cells and investigate its role in dysplastic brain tissue.
Snippet: …RC220377 (OriGene Technologies, Rockville, MD, USA) cloned in a pCMV6-AC-HA vector (OriGene Technologies, cat. PS100004). … RC220377 (OriGene Technologies, Save Cached
FigureFigureFigure
Deleterious Rare Mutations of GLI1 Dysregulate Sonic Hedgehog Signaling in Human Congenital Heart Disease
Peng, Li, Chen et al
Front Cardiovasc Med (2022) 9, 798033 DOI: 10.3389/fcvm.2022.798033
Cited by 8
AI Summary: In this study, the pCMV6-AC-HA expression vector from OriGene (#PS100004) was used to subclone GLI2 for functional analyses of GLI1 mutations implicated in congenital heart disease.
Snippet: …GLI2 was subcloned into SgfI/NotI restriction enzyme sites of pCMV6-AC-HA (Origene, #PS100004). … We then performed the site-directed mutagenesis using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, #210518) accordi;GLI2 was subcloned into SgfI/NotI restriction enzyme sites of pCMV6-AC-HA (Origene, #PS100004). …
FigureFigureFigure
Protein S-glutathionylation stimulate adipogenesis by stabilizing C/EBPβ in 3T3L1 cells
Watanabe, Watanabe, Fujioka et al
FASEB J. (2020)
AI Summary: OriGene products, including the Myc-FLAG-tagged mouse C/EBPβ plasmid (MR227563), Myc-FLAG-tagged mouse PIAS1 plasmid (MR209770), and C-terminal HA-tag vector (PS100004), were used to express and study the roles of C/EBPβ and PIAS1 in the investigation of protein S-glutathionylation during adipogenesis.
Snippet: Cambridge, UK). Myc‐FLAG‐tagged mouse C/EBPβ plasmid (MR227563), Myc ‐FLAG‐tagged mouse PIAS1 plasmid (MR209770), and C‐terminal HA‐tag vector (PS100004) were from Origene (Rockville, MD, USA). OxiSelect
Von Willebrand factor A domain-containing protein 8 (VWA8) localizes to the matrix side of the inner mitochondrial membrane
Luo, Ma, Sand et al
Biochem. Biophys. Res. Commun. (2019) 521 (1), 158-163 DOI: 10.1016/j.bbrc.2019.10.095
AI Summary: OriGene’s pCMV6-AC-HA plasmid (#PS100004) was used to generate an expression construct for enhanced green fluorescent protein (eGFP), enabling the study of VWA8 protein localization in this research.
Snippet: … A cDNA fragment coding for enhanced green fluorescent protein (GFP), ATG-TCCGGA-eGFP, was inserted into a plasmid, pCMV6-AC-HA (Origene #PS100004), to generate the pCMV-SG-eGFP expression construct. … A cDNA fragment coding for enhanced green fluorescent protein (GFP), ATG-TCCGGA-eGFP, was inserted into a plasmid, pCMV6-AC-HA (Origene #PS100004), to generate the pCMV-SG-eGFP expression construct. …
Showing 1-6 of 11 papers.
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