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pCMV6-AC-HA-His Mammalian Expression Vector

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SKU
PS100008
pCMV6-AC-HA-His, mammalian vector with C-terminal HA-His tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag HA-His
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal HA-His tags.
  • Such clones are the best for detection and purification of the transgene expression using anti-HA or anti-His antibodies or a nickel affinity columns
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal HA-His-tagging vector, PS100017.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
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eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR
Wilczynska, Gillen, Schmidt et al
Genome Biol. (2019) 20 (1), 262 DOI: 10.1186/s13059-019-1857-2
Cited by 78
AI Summary: OriGene’s C-terminal flag-myc tagged eIF4A1 plasmid was used to generate N-terminal His-HA-tagged eIF4A1 constructs for functional studies in this paper.
Snippet: 2. pRL-SV40 plasmid used in luciferase assay experiments was described previously [11]. N-terminal His-HA-tagged eIF4A1 and eIF4A2 were generated by excising either C-terminal flag-myc tagged eIF4A1 (Origene Inc.) or untagged eIF4A2 described previously [11] with AsiSI and NotI (NEB) restriction enzymes. The excised product was then gel purified and re-ligated into the N-terminal His-HA tagged pC
FigureFigureFigure
Decapping protein EDC4 regulates DNA repair and phenocopies BRCA1
Hernández, Ramírez, Minguillón et al
Nat Commun (2018) 9 (1), 967 DOI: 10.1038/s41467-018-03433-3
AI Summary: OriGene’s pCMV6-EDC4-HA-HIS plasmid (PS100008) was used in this study to express EDC4 for functional analysis of its role in DNA repair and its interaction with the BRCA1 pathway.
Snippet: ls. After 4 h, the medium was removed and fresh complete DMEM medium was added. Cells were allowed to grow for 48 h before analysis. Plasmid construction The pCMV6-EDC4-HA-HIS was purchased from OriGene (PS100008). The fragment containing EDC4-HA-HIS was extracted by digesting with AsiSI and PmeI and then cloned into pGK lentiviral vector modified to contain AsiSI and PmeI target sequences. T
FigureFigureFigure
Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin
Izumi, Nakato, Zhang et al
Nat. Genet. (2015) 47 (4), 338-44 DOI: 10.1038/ng.3229
Cited by 149
AI Summary: OriGene products contributed to this study by providing Myc-DDK-tagged AFF4 (RC217692) and Myc-DDK-tagged SIAH1a (RC206576) plasmids, as well as the RapidShuttling kit (PS200008) used for generating tagged constructs, which facilitated functional analyses of the implicated proteins in the described developmental syndrome.
Snippet: … Antibodies to ELL2 used for immunoblotting (A302-505A) and AFF4 used for ChIP-seq analysis (A302-538A) were purchased from Bethyl Lab. Antibodies to NIPBL (sc-374625) and cyclin T1 (sc-10750) were from Santa Cruz Biotechnology. …Plasmids encoding MycDDK-tagged AFF4 (RC217692) and Myc-DDK-tagged SIAH1a (RC206576) were obtained from OriGene Technologies. To generate HA-His-tagged SIAH1a, the SIAH1A cDNA was subcloned into pCMV6-AC-HA-His, using the OriGene RapidShuttling kit (SfgI-MluI, PS200008) according to the manufacturer’s directions. Mutagenesis of the AFF4 expression plasmid to introduce each of the nonsynonymous variants was performed using the QuikChange Site-Directed Mutagenesis kit by Agilent Technologies following the manufacturer’s protocol. …
A Targeting Modality for Destruction of RNA Polymerase I that Possesses Anticancer Activity
Peltonen, Colis, Liu et al
Cancer Cell (2014) 25 (1), 77-90 DOI: 10.1016/j.ccr.2013.12.009
Cited by 324
AI Summary: OriGene products contributed to this study by providing the human USP36-myc plasmid and the pCMV6-AC-HA-His vector used to construct the RPA194-HA expression plasmid, both of which were essential for investigating the molecular mechanisms underlying BMH-21’s anticancer activity.
Snippet: … Human USP36-myc was obtained from Origene and pCIneo-HAUSP-Flag was kindly provided by Dr. B. Vogelstein (Johns Hopkins University). …RPA194-HA was constructed by cloning full-length human RPA194 in pCR-4-TOPO-RPA194 (Invitrogen) to pCMV6-AC-HA-His vector (Origene) to yield pRPA194-HA. 12H-Benzo[g]pyrido[2,1-b]quinazoline-4-carboxamide, N-[2(dimethylamino) ethyl]-12-oxo (BMH-21) was obtained from ChemDiv, verified for purity using liquid chromatography/mass spectrometry, and 1 H-NMR
Regulation of Constitutive GPR3 Signaling and Surface Localization by GRK2 and β-arrestin-2 Overexpression in HEK293 Cells
Lowther, Uliasz, Götz et al
PLoS ONE (2013) 8 (6), e65365 DOI: 10.1371/journal.pone.0065365
Cited by 25
AI Summary: OriGene contributed to this study by providing the Human GPR3 plasmid (pCMV6-AC-HA-His, GPR3-HA), which was used for exogenous expression and analysis of GPR3 localization and signaling in HEK293 cells.
Snippet: provided by Y. Saeki (Ohio State University) in pHGCX Human GPR3 in pCMV6-AC-HA-His (GPR3-HA) was purchased from Origene (Rockville, MD). ... Films were scanned and band intensities were analyzed by densitometry using Image J software (National Institutes of Health).
Showing 1-5 of 5 papers.
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