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pCMV6-AC-DDK Mammalian Expression Vector

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SKU
PS100005
pCMV6-AC-DDK, mammalian vector with C-terminal DDK tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed as a tagged protein with a C-terminal DDK-tag. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene expression using an anti-DDK antibody.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal DDK-tagging vector, PS100014.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
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SIFD-associated TRNT1 deficiency unveils importance of TSPO during macrophage antibacterial and antiviral responses
Ahmed, Slade, Fatica et al
Front Immunol (2025) 16, 1497766 DOI: 10.3389/fimmu.2025.1497766
Cited by 1
AI Summary: OriGene products, specifically the plasmid encoding mouse TSPO (Cat#: MC207241) and the empty mammalian plasmid (PS100005), were used for TSPO overexpression and control experiments to assess the role of TSPO in rescuing macrophage inflammatory responses in TRNT1-deficient cells.
Snippet: tained from Qiagen. PK11195 (Cat#: 10191-264) was purchased from BioVision. A plasmid encoding mouse TSPO coding sequence (Cat#: MC207241) and an empty mammalian plasmid (PS100005) were received from OriGene. Animals and macrophage cell culture All animal procedures were approved by the Carleton University Animal Care Committee and were conducted in accordance with the guidelines provided by the C
Ultrarare Loss-of-Function Mutations in the Genes Encoding the Ionotropic Glutamate Receptors of Kainate Subtypes Associated with Schizophrenia Disrupt the Interaction with PSD95
Hu, Wu, Hsu et al
J Pers Med (2022) 12 (5) DOI: 10.3390/jpm12050783
Cited by 13
AI Summary: OriGene products, including pCMV6-Myc-DDK-tagged GRIK1 (RC222898), GRIK2 (RC222369), DLG4 (RC215178) plasmids, and the pCMV6-Myc-DDK and pCMV6-AC-GFP vectors, were used to express and study the functional effects of mutant and wild-type kainate receptor genes in cell-based assays for this schizophrenia research.
Snippet: … Gene Constructs and Transient Transfection of Cell Lines pCMV6-Myc-DDK-tagged GRIK1 (RC222898), GRIK2 (RC222369), and DLG4 genes (RC215178) were purchased from Origene Technologies, Inc., Rockville, MD, USA. The cDNA encoding GRIK3, GRIK4, and GRIK5 were synthesized by MDBio, Inc. …
RTEL1 influences the abundance and localization of TERRA RNA
Ghisays, Garzia, Wang et al
Nat Commun (2021) 12 (1), 3016 DOI: 10.1038/s41467-021-23299-2
Cited by 56
AI Summary: In this study, OriGene products—including RTEL1 shRNA (OriGene, TL313374) and RTEL1 expression plasmid (OriGene, RC221408)—were used to manipulate RTEL1 levels in human cells, enabling the authors to investigate how RTEL1 affects the abundance and localization of TERRA RNA.
FigureFigureFigure
TRAP1 chaperone protein mutations and autoinflammation
Standing, Hong, Paisan-Ruiz et al
Life Sci Alliance (2020) 3 (2) DOI: 10.26508/lsa.201900376
Cited by 17
AI Summary: OriGene’s pCMV6-AC-DKK vector was used to overexpress wild-type and mutant (R128H) TRAP1 in THP1 cells, enabling functional studies of TRAP1 mutations in the context of autoinflammation.
Snippet: as changed to RPMI with 2% FCS. After a further 24 h, the cells were ready for further experiments. Overexpression of TRAP1 1 million THP1 cells were transfected with 0.5 μg of pCMV6-AC-DKK vector (OriGene) containing wild-type or R128H TRAP1, or empty vector using the Amaxa nucleofector following the manufacturer’s protocol for kit V. Mutated TRAP1 was produced using Q5-site directed mutagene;;....
GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease
Chuang, Tsai, Hsueh et al
Sci Adv (2018) 4 (9), eaat5401 DOI: 10.1126/sciadv.aat5401
AI Summary: OriGene products, specifically the pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, and pCMV6-AC-Flag vectors, were used to construct plasmids for expressing tagged proteins (such as PKCθ and AhR) that enabled the study of protein interactions and signaling mechanisms underlying IL-17A induction in autoimmune disease.
Snippet: PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …PKCθ, HA-tagged AhR, and Flag-tagged PKCθ plasmids were constructed by subcloning individual complementary DNAs into pCMV6-AC-CFP, pCMV6-AC-YFP, pCMV6-AC-Myc, pCMV6-AC-HA, or pCMV6-AC-Flag vector (OriGene Technologies). …
FigureFigureFigure
Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency
Boutboul, Kuehn, Van de Wyngaert et al
J. Clin. Invest. (2018)
AI Summary: OriGene’s pCMV6-AC-DDK mammalian expression vector was used to subclone IKZF1 cDNA for functional studies of IKZF1 mutations in this paper.
Snippet: ctions, and infections. Human IKAROS family zinc finger 1 (IKAROS) (_IKZF1_;NM_006060) cDNA was synthesized (GeneScript) and subcloned into the mammalian expression vector pcDNA3-HA or pCMV6-AC-DDK (Origene), pcDNA3.1D/V5-His-TOPO (Invitrogen). Indicated mutants for _IKZF1_ were generated based on the site-directed mutagenesis protocol with AccuPrime Pfx DNA Polymerase (Thermo Fisher Scientific)
Showing 1-6 of 13 papers.
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