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pCMV6-AN-DDK Mammalian Expression Vector

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SKU
PS100014
pCMV6-AN-DDK, mammalian vector with N-terminal DDK tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
N-Tag DDK
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal DDK-tag. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene expression using an anti-DDK antibody.
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal DDK-tagging vector, PS100005
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Novel biomimetic mesoporous silica nanoparticle system possessing targetability and immune synergy facilitates effective solid tumor immuno-chemotherapy
Zhu, Liu, Yi et al
Biomater Adv (2023) 144, 213229 DOI: 10.1016/j.bioadv.2022.213229
Cited by 21
AI Summary: OriGene’s pCMV6-AN-DDK (PS100014) mammalian expression vector was used to clone the chimeric anti-HER2 scFv-LFA3 cDNA, enabling the production of targeting molecules critical for the biomimetic nanoparticle system developed in this study.
Snippet: [37]. Chimeric anti-Her2 scFv-LFA3 cDNA was cloned into pCMV6-AN-DDK (PS100014, Origene, USA), a mammalian expression vector with an N-terminal DDK tag. T
Loss-of-function or gain-of-function variations in VINCULIN (VCL) are risk factors of human neural tube defects
Wang, Qin, Peng et al
Mol Genet Genomic Med (2021)
Cited by 4
AI Summary: OriGene products contributed to this study by providing the human VCL (NM_003373) cDNA clone and the anti-GFP antibody (TA150041), which were used for functional analyses and protein detection of VCL variants associated with neural tube defects.
Snippet: The human VCL (NM_003373) cDNA clone was purchased from OriGene Technologies (Beijing, China) and inserted into the mammalian expressing plasmid pCMV6-AN-DDK the anti-Flag antibody (CST, 8146S) or anti-GFP antibody (OriGene, TA150041);- The human VCL (NM_003373) cDNA clone was purchased from OriGene Technologies (Beijing, China) and inserted into the mammalian expressing plasmid pCMV6-AN-DDK the anti-Flag antibody (CST, 8146S) or anti-GFP antibody (OriGene, TA150041)
Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice
Wada, Kikuchi, Kaga et al
PLoS Genet. (2020) 16 (2), e1008628 DOI: 10.1371/journal.pgen.1008628
Cited by 38
AI Summary: In this study, OriGene's pCMV6-AN-DDK (PS100014) vector was used to subclone and express wild-type and mutant human LSS cDNAs, enabling functional analysis of LSS mutations identified in patients.
Snippet: S cDNAs (NM_002340.5, carrying SNP rs2254422 and rs2254524) were prepared by RT-PCR with RNA from the blood of the patients and their family members. cDNAs were subcloned into pCMV6-AN-DDK (PS100014, OriGene, Rockville, MD, USA). … Cell culture and transfection HeLa cells (RCB0007, provided by the RIKEN BRC through the National BioResource Project o;Wild-type, R234fs, and R177Q human LSS cDNAs (NM_002340.5, carrying SNP rs2254422 and rs2254524) were prepared by RT-PCR with RNA from the blood of the patients and their family members. cDNAs were subcloned into pCMV6-AN-DDK (PS100014, OriGene, Rockville, MD, USA). …
Dissociating STAT4 and STAT5 Signaling Inhibitory Functions of SOCS3: Effects on CD8 T Cell Responses
Hwang, Holland, Valenteros et al
Immunohorizons (2019) 3 (11), 547-558 DOI: 10.4049/immunohorizons.1800075
Snippet: JAK1 and JAK2 cDNA were subcloned into an expression vector (pCMV6-AN-DDK) that has built-in DDK sequence in the N terminus (Origene Technologies, Rockville, MD). … The sequences of the primers used for JAK1 were 5′-GCCGGCGCGCCcatgcagtatctaaatataaaag-3′ and 5′
Biallelic GALM pathogenic variants cause a novel type of galactosemia
Wada, Kikuchi, Arai-Ichinoi et al
Genet Med (2019) 21 (6), 1286-1294 DOI: 10.1038/s41436-018-0340-x
Cited by 59
AI Summary: OriGene products, specifically the TrueORF cDNA clones and anti-GALM antibody (TA501348), were used in this study for in vitro GALM expression and immunoblot assays to assess the presence and stability of GALM protein variants in patients with galactosemia.
DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection
Pattabhi, Knoll, Gale et al
J. Interferon Cytokine Res. (2019) 39 (6), 331-346 DOI: 10.1089/jir.2018.0163
AI Summary: OriGene products, specifically the DHX15 cDNA (RC205914) and expression vectors pCMV6 (PS100012 and PS100014), were used to generate tagged DHX15 proteins for functional studies in this paper investigating DHX15's role in antiviral immune signaling.
Snippet: kit (Stratagene). DHX15 complementary DNA (cDNA;RC205914;OriGene) was cloned into vectors pEFTak and pCMV6 (PS100012 or PS100014;OriGene) for expression as N-terminally Flag- or myc-tagged proteins. DHX15;- kit (Stratagene). DHX15 complementary DNA (cDNA
Showing 1-6 of 23 papers.
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