pCMV6-AC-His Mammalian Expression Vector
$670.00
In Stock*
Specifications
| Product Data | |
| C-Tag | His |
|---|---|
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Expression Promoter | CMV |
| Features |
|
| Download Vector Sequence | |
| Schematic of the multiple cloning sites |
|
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Heparin-binding of the human chitinase-like protein YKL-40 is allosterically modified by chitin oligosaccharides
Magnusdottir, Thormodsson, Kjalarsdottir et al
Biochemistry and Biophysics Reports (2025) 41, 101908-101908 DOI: 10.1016/j.bbrep.2024.101908
Magnusdottir, Thormodsson, Kjalarsdottir et al
Biochemistry and Biophysics Reports (2025) 41, 101908-101908 DOI: 10.1016/j.bbrep.2024.101908
Cited by 3
AI Summary: OriGene contributed to the study by providing the human YKL-40 cDNA, TransPass D2 Transfection Reagent, plasmid vector pCMV6-AC-His (PS100002), and Rapid Shuttling kit, which were used for cloning, expressing, and transfecting YKL-40 in cell lines.
Snippet: A human embryonic kidney cell line (HEK293) was from ECACC, UK. A human fetal lung fibroblast cell line (HFL-l) was from ATCC, USA. The human YKL-40 cDNA, TransPass D2 Transfection Reagent, plasmid vector pCMV6-AC-His (PS100002) containing a C-terminal 6x Histidine tag and a Neomycin resistance gene, and Rapid Shuttling kit, were from OriGene, USA
A novel model of nephrotic syndrome results from a point mutation in Lama5 and is modified by genetic background
Falcone, Nicol, Blease et al
Kidney Int (2021) 101 (3), 527-540 DOI: 10.1016/j.kint.2021.10.031
Falcone, Nicol, Blease et al
Kidney Int (2021) 101 (3), 527-540 DOI: 10.1016/j.kint.2021.10.031
Cited by 10
AI Summary: OriGene's anti-6XHis antibody was used in this study to detect His-tagged proteins during immunoblotting, enabling analysis of LAMA5 protein expression and assembly in the novel nephrotic syndrome mouse model.
Snippet: … After blocking with 5% w/v milk, membranes were incubated with anti-6XHis antibody (Origene;1:1000) overnight. Goat–anti-rabbit IRDye680LT (1:15,000;LI-COR Biosciences) was used as secondary antibody, and fluorescent blots were scanned using a LI-COR Odyssey SA scanner (LI-COR Bi;membranes were incubated with anti-6XHis antibody (Origene;1:1000) overnight
Alternative splicing of the TNFSF13B (BAFF) pre-mRNA and expression of the BAFFX1 isoform in human immune cells
Stelmach, Pütz, Pollmann et al
Gene (2020) 760, 145021 DOI: 10.1016/j.gene.2020.145021
Stelmach, Pütz, Pollmann et al
Gene (2020) 760, 145021 DOI: 10.1016/j.gene.2020.145021
Cited by 7
AI Summary: OriGene’s pCMV6-AC-His vector (#PS100002) was used in this study to clone and express the BAFFX1 isoform, enabling the investigation of its role in human immune cells.
Snippet: Full length encoding BAFFX1 cDNA was ligated into pCMV6-AC-His vector (#PS100002, OriGene Technologies) over artificial restriction sites Sgf I (AsiS I) and Mlu I. For generation of expression construct MBP-rsBAFF X1, the BAFFX1 extracellular part was amplified
Phillygenin, a MELK Inhibitor, Inhibits Cell Survival and Epithelial-Mesenchymal Transition in Pancreatic Cancer Cells
Li, Chen, Yang et al
Onco Targets Ther (2020) 13, 2833-2842 DOI: 10.2147/OTT.S238958
Li, Chen, Yang et al
Onco Targets Ther (2020) 13, 2833-2842 DOI: 10.2147/OTT.S238958
AI Summary: In this study, OriGene's p-CMV6-MELK and p-CMV6-control plasmids were used to manipulate MELK expression in pancreatic cancer cells, enabling the researchers to assess the role of MELK in the anti-cancer effects of phillygenin.
Snippet: PHI was obtained from the National Institutes for Food and Drug Control (China, Figure 1A). PHI was dissolved in 0.1% DMSO with 0.1% DMSO as vehicle control. Antibodies against MELK, Vimentin, E-cadherin, and GAPDH were procured from Cell Signaling Technology (Danvers, MA). p-CMV6-MELK and p-CMV6-control plasmids were purchased from OriGene (USA). MELK siRNA was purchased from GenePharma (China)
Recombinant annexin A6 promotes membrane repair and protects against muscle injury
Demonbreun, Fallon, Oosterbaan et al
J. Clin. Invest. (2019)
Demonbreun, Fallon, Oosterbaan et al
J. Clin. Invest. (2019)
AI Summary: OriGene's First-strand cDNA Synthesis Kit was used in this study to generate cDNA from extracted RNA, enabling subsequent RT-qPCR analysis of gene expression related to annexin A6's role in membrane repair and muscle protection.
Snippet: RNA was extracted using the QIAGEN RNeasy Mini Kit. 1 μg of total RNA was used to synthesize complementary DNA (cDNA) with First-strand cDNA Synthesis Kit (OriGene). RT-qPCR and gene expression analysis was carried out as described (Vardabasso et al., 2015). PCR amplification was performed using
In Vitro Assays to Measure the Membrane Tethering and Lipid Transport Activities of the Extended Synaptotagmins
Bian, De Camilli
Methods Mol. Biol. (2019) 1949, 201-212 DOI: 10.1007/978-1-4939-9136-5_15
Bian, De Camilli
Methods Mol. Biol. (2019) 1949, 201-212 DOI: 10.1007/978-1-4939-9136-5_15
Snippet: 2 Materials. 2.1 Expression and Purification of E-Syt1 cyto. 1. Plasmids: pCMV6-AN-His vector (eg, OriGene) containing the region coding for residues 93-1104 of human E-Syt1 (see [5, 10] for detailed information for making this construct). 2. Expi293F cells
Showing 1-6 of 19 papers.
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