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pEX-C-GST Bacterial Expression Vector

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SKU
PS100083
pEX-C-GST, bacterial expression vector with C-terminal GST tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag GST
E. coli Selection Ampicillin (100 ug/mL)
Features

This vector is for E.coli expression.

  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal GST tag.
  • Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector.
  • Detection of the transgene is best done using an anti-GST antibody.
  • If the C-terminal tagging interferes with the protein's function, you can choose the N-terminal GST-tagging vector, PS100029
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome
Asamitsu, Yabuki, Ikenoshita et al
Sci Adv (2021) 7 (3) DOI: 10.1126/sciadv.abd9440
Cited by 104
AI Summary: In this study, OriGene’s pCMV6-Entry-FMR1 plasmid was used to express FMRpolyG protein, enabling the investigation of its interaction with CGG repeat RNA G-quadruplexes and its role in neuronal dysfunction in fragile X-related tremor/ataxia syndrome.
FigureFigureFigure
Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts
Bochler, Querido, Prilepskaja et al
Cell Rep (2020) 33 (12), 108534 DOI: 10.1016/j.celrep.2020.108534
Cited by 38
AI Summary: OriGene’s pEX-C-GST plasmid (Cat#PS100083) was used in this study to generate GST fusion proteins for pull-down assays, supporting the investigation of structural differences in translation initiation between trypanosomatids and mammalian hosts.
Snippet: pGL4-CMV Wagner et al., 2014 N/A pFASTBAC1-eIF3k Fraser et al., 2004 N/A pFASTBAC1-eIF3d Fraser et al., 2004 N/A pFASTBAC1-eIF3e Fraser et al., 2004 N/A pEX-C-GST OriGene Cat#PS100083 ------------------------- Oligonucleotides ------------------------- Primers for cloning used in this study, see List of primers (Supplemental Information) This paper N;pEX-C-GST OriGene Cat#PS100083
Showing 1-2 of 2 papers.
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