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pEX-N-GST Bacterial Expression Vector

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SKU
PS100029
pEX-N-GST, bacterial expression vector with N-terminal GST tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
N-Tag GST
E. coli Selection Ampicillin (100 ug/mL)
Features
  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal GST tag.
  • Detection and purification of the transgene are best done using a glutathione affinity column.
  • If the N-terminal tagging interferes with the protein's function, you can choose the C-terminal GST-tagging vector, PS100083
  • PS100028_F Primer can be used to sequence the target sequence after cloning.
  • PS100028_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor
Cho, Ju, Seo et al
Int J Mol Sci (2021) 22 (22) DOI: 10.3390/ijms222212194
Cited by 3
AI Summary: OriGene's pEX-N-GST vector was used to clone hNME1 and related domains with an N-terminal GST tag, enabling the study of their interactions and functions in neuronal differentiation of porcine mesenchymal stem cells.
Snippet: and domains thereof were cloned along with a N-terminal GST tag into the pEX-N-GST vector (OriGene, Rockville, MD,
FigureFigureFigure
Characterisation of three novel β-1,3 glucanases from the medically important house dust mite Dermatophagoides pteronyssinus (airmid)
Waldron, McGowan, Gordon et al
Insect Biochem. Mol. Biol. (2019) 115, 103242 DOI: 10.1016/j.ibmb.2019.103242
AI Summary: OriGene's pEX-N-GST Precision Shuttle vector was used to clone gene fragments encoding the novel β-1,3 glucanases, enabling their recombinant expression and purification for biochemical characterization in this study.
Snippet: S3 Data). Gene fragments were cloned into the pEX-N-GST Precision Shuttle vector (OriGene), recombinantly expressed in and purified from E. coli according to methods described by Dolan et al. (2014). Recombinant proteins
HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma
Xu, Tu, Dou et al
Mol. Cancer (2017) 16 (1), 178 DOI: 10.1186/s12943-017-0748-y
AI Summary: OriGene’s GST-HSP90 protein was used in GST-pulldown assays to investigate and confirm the interaction between HSP90 and PKM2 in hepatocellular carcinoma cells.
Snippet: GST-HSP90 or control GST (OriGene Technologies, Inc., Rockville, MD, USA) was added into the cell lysates harvested from the cells transfected with HA-tagged PKM2. After being incubated with Glutathione beads (Sigma-Aldrich, St Cached
Structural, mechanistic and functional insight into gliotoxin bis-thiomethylation in Aspergillus fumigatus
Dolan, Bock, Hering et al
Open Biol (2017) 7 (2) DOI: 10.1098/rsob.160292
AI Summary: OriGene’s pEX-N-GST-GtmA expression plasmid was used to produce recombinant GtmA protein in E. coli, enabling the structural and functional studies described in the paper.
Snippet: Expression plasmids pEX-N-GST-GtmA (OriGene) and pET19 m-GtmA (modified pET19b, Novagen) were freshly transformed into chemically competent Escherichia coli BL21 (DE3) cells and subsequently used for pre-culture inoculation in lysogenic broth (LB) supplemented with 100 mg l 2 1 ampicil- lin. Large-scale production of the native protein was carried out in terrific broth including the required ...
A Mutation in NPAS3 That Segregates with Schizophrenia in a Small Family Leads to Protein Aggregation
Nucifora, Wu, Lee et al
Mol Neuropsychiatry (2016) 2 (3), 133-144 DOI: 10.1159/000447358
Cited by 28
AI Summary: OriGene products, specifically the NPAS3 cDNA clone (OriGene, SC119515) and anti-NPAS3 antibody (OriGene, TA324113), were used in this study to express and detect NPAS3 protein variants, enabling the investigation of how the V304I mutation promotes protein aggregation associated with schizophrenia.
Single particle fluorescence burst analysis of epsin induced membrane fission
Brooks, Shoup, Kustigian et al
PLoS ONE (2015) 10 (3), e0119563 DOI: 10.1371/journal.pone.0119563
Cited by 8
AI Summary: OriGene’s pEX-N-His6-GST vector was used to sub-clone and express full-length rat epsin in E. coli, enabling the study of epsin-induced membrane fission in this paper.
Snippet: DTT. The coding sequence of full-length rat epsin was obtained from Addgene (pCDNA3.1-Epsin1;plasmid 22225) and was sub-cloned into the pEX-N-His 6 -GST vector (Origene) for expression in E. coli BL21[DE3]. Purification ... Cite SaveSaving
Showing 1-6 of 9 papers.
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