TissueScan, Lung Cancer cDNA Array V

Snippet: obtained from all patients and the protocol used was in accordance with the Declaration of 2 Helsinki. Human lung cancer cDNA array (# HLRT105) was purchased from OriGene 3 (Rockville). 4 5 Statistics 6 A One-way ANOVA and Tukey’s test for post-hoc analysis and Student’s t tests were 7 performed using Prism version 5 (GraphPad Software). …

Snippet: …Quantitative PCR was performed using an ABI 7300 real-time PCR system (Applied Biosystems), and gene expression was calculated using the 2−ΔCt or 2−ΔΔCt methods with calibration samples included in each experiment. For lung cancer tissue analysis, a TissueScan cDNA array plate (HLRT105) was purchased from Origene (Rockville, MD, USA), which consisted of seven-normal, six-stage IA, 5-IB, 13-IIB, 7-IIIA, 7-IIIB, and 3-IV. …

Snippet: The aberrant expression of claudins (CLDNs), which are tight junctional proteins, is seen in various solid tumors, but the regulatory mechanisms and their pathophysiological role are not well understood. Both CLDN1 and CLDN11 were highly expressed in human lung;... chnologies, Santa Clara, CA, USA, and BioChain Institute, Hayward, CA, USA). The expression of CLDNs between human normal lung and SCC...

Snippet: The lung cancer cDNA arrays (HLRT101, HLRT103, and HLRT105) were from OriGene. Lung cancer tissue arrays were from Super Bio-Chips (CC5). Blood buffy coat cellular RNAs from 50 healthy people and 49 lung cancer patients were obtained from NRPB lung cancer tissue bank, which obtained informed consent from patients