TissueScan, Colon Cancer cDNA Array I

Snippet: …Immunohistochemistry demons;Samples from human colon carcinoma patients were obtained from five commercial arrays (TissueScan cDNA arrays HCRT301, HCRT302, HCRT303, HCRT304, HCRT305, OriGene, Rockville, Maryland, USA)

Snippet: Samples from human colon carcinoma patients were obtained from ve commercial arrays (TissueScan cDNA arrays HCRT301, HCRT302, HCRT303, HCRT304, HCRT305, OriGene, Rockville, Maryland, USA). Samples in which mRNA levels for housekeeping genes were not detectable were excluded from the analysis. A total of 158 patients were analyzed and subdivided according to the

Snippet: The mRNA levels of DJ-1 were higher in CRC samples than that in normal mucosal tissues (Supplementary Fig. 1A). Consistently, elevated DJ-1 mRNA was also observed in CRC tissues using qRT-PCR in an OriGene Colon Cancer cDNA Array (Fig. 1a, P = 0.016)

Snippet: DuoLink in situ fluorescence reagents were purchased from Sigma-Aldrich. The TissueFocus Colon Cancer Tissue Microarray was purchased from OriGene Technologies (Rockville, USA)