TissueScan, Colon Cancer cDNA Array IV

Snippet: Colon cancer–staged tumor panel cDNA was purchased from OriGene (cat. #HCRT104). For normal colon tissue used in the colorectal cancer cell panel, normal tissue cDNA was obtained from OriGene and normalized to GAPDH for analysis. For all other RNA analysis, samples were normalized to actin

Snippet: was purchased from Origene (Cat#: HCRT104). For normal colon tissue used in the CRC cell array, normal tissue cDNA was obtained from Origene and normalized to

Snippet: d 119 individuals (age range, 26-76 years;67 males and 52 females) following a colonoscopy at Cathay Healthcare Management (Taipei City, Taiwan) were collected. In addition, one cDNA array (HCRT104;OriGene Technologies, Inc.) of 48 colon tissues covering non-CRC status (n=8) and four CRC stages (n=40) was purchased to examine the predictive model. LOOCV, discriminant analyses and predictive mod

Snippet: Long non-coding RNA (lncRNA) DANCR has been reported to participate in key processes such as stem cell differentiation and tumorigenesis. In a high throughput screening for lncRNAs involved in Doxorubicin-induced apoptosis, we found DANCR was suppressed

Snippet: 8;cat. no. HCRT104;OriGene Technologies, Inc.). Total RNA from the cultured cells was extracted using the Easy Pure Total RNA Mini kit (Bioman Scientific Co. Ltd.), according to the manufacturer's protocol. Subsequently, total;- 8