TissueScan, Crohn's/Colitis cDNA Array II

Snippet: …Herein, using wild-type and GPR4 knockout mouse CAC models, we investigated the role of GPR4 in inflammation-dr;Real-time PCR was performed as previously described [16]. Crohn’s and ulcerative colitis cDNA arrays were purchased from Origene Technologies (catalog #CCRT102, Rockville, MD) and utilized in real-time PCR analysis using specific primer-probes for human GPR4, TNF-α, IFN-γ and β-actin [23,31]

Snippet: Crohn's and colitis cDNA array were purchased from Origene Technologies (Catalog #CCRT102, Rockville, MD) to assess GPR65 gene

Snippet: Page 9. (Hs002692477_s1) (41). Crohn's and colitis cDNA array was purchased from Origene Technologies (Catalog #CCRT102, Rockville, MD) to assess TDAG8 gene expression in human inflammatory bowel disease lesions compared to non-inflamed intestinal View as HTML

Snippet: Article.;TissueScan Crohn’s and Colitis tissue cDNA array plates (#CCRT101 and #CCRT102) of colon and ileum were purchased from Origene Inc. PCR was performed after addition of master mix and primers as described previously26

Snippet: Crohn’s and colitis cDNA arrays were purchased from Origene Technologies (catalog #CCRT102, Rockville, MD) and subjected to real-time PCR using specific primer-probes for human GPR4 and β-actin. The primer and probe used for human GPR4 has been previously described [16]. The cDNA array contained 47 samples including 7 normal, 14 Crohn’s, and 26 ulcerative colitis intestinal samples from patients diagnosed with IBD. …

Snippet: Deidentified human intestinal tissue cDNA was obtained from the TissueScan Crohn’s/ColitisII array (OriGene Technologies, Rockville, MD). Complete patient/sample characteristics can be accessed from the supplier’s website (www.origene.com)