Parp1 Mouse shRNA Plasmid (Locus ID 11545)

Snippet: DY2230AA Addgene Cat#64878 PARP1 mouse shRNA Origene Cat#TL509117 Not available PARP1 mouse ORF Origene Cat# MR211449L4 Not available HUWE1 mouse shRNA Origene Cat#TL503398 Not available MSH2 mouse shRNA Dharmacon Cat# V3SM11241- 234501644 Not available UBE2O mouse siRNA Dharmacon Cat#L-064151-01-0020, (J-064151-09, J0654151-10,J-065

Snippet: … Following uptake, some endosomal vesicles are transported to lysosomes for degradation, while the others are returned to the plasma membrane. The destination of these vesicles is d;- shRNA plasmids targeting human or mouse PARP1 or scrambled shRNA (Origene) were introduced into NIH3T3 and HeLa cells using Lipofectamine 3000 transfection Save Cached

Snippet: … Plasmids shRNA targeting PARP1 and GFP-PARP1 were purchased from Origene. PARP1 D214-GFP and PARP1 E988K-GFP were generated with primers (5′-TGGGAGGAGTGGATGAAGTGG-3′ and 5′-CCTCATCGCCTTTTCTCTTTCCTTC-3′) and (5′-AAGTACATTGTCTATGATATTGC-3′ and 5′-GTTATA;- goat anti-mouse and rabbit immunoglobulin from Thermo Fisher Scientific. Plasmids shRNA targeting PARP1 and GFP-PARP1 were purchased from Origene. PARP1 D214-GFP, PARP1 E988K-GFP were generated with primers

Snippet: PARP-1 HuSH cells were created by transfecting HaCaT cells with PARP-1 shRNA (Origene;HuSH 29-mer). ... on retention of UVR-induced photoproducts in cancer-relevant target cells, immortalized human keratinocytes (HaCaT) were transfected with a short hairpin RNA

Snippet: Site-directed Mutagenesis and Transfections. A PARP-1 expression plasmid was purchased from OriGene Technologies, Inc. (Rockville, MD), and site-directed mutagenesis of the first and second zinc fingers of PARP-1 was performed by Retrogen, Inc. (San Diego, CA).