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Map2k1 Mouse shRNA Plasmid (Locus ID 26395)

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SKU
TL502589
Map2k1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
2 Weeks*

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Specifications
Specifications
Product Data
Locus ID 26395
Synonyms MAPKK1; Mek1; MEKK1; Prkmk1
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components Map2k1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 26395). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC054754, NM_008927, NM_008927.1, NM_008927.2, NM_008927.3, BC051137, NM_008927.4
UniProt ID P31938
Summary Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.UniProtKB/Swiss-Prot Function
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

The epithelial polarity regulator LGALS9/galectin-9 induces fatal frustrated autophagy in KRAS mutant colon carcinoma that depends on elevated basal autophagic flux
Wiersma, De Bruyn, Wei et al
Autophagy (2015)
Cited by 58
AI Summary: OriGene products, specifically siRNAs targeting BRAF (SR300470), RAF1/CRAF (SR303972), MAP2K1 (SR303761), BECN1 (SR305711), and ATG5 (SR306286), as well as an anti-BECN1 antibody (TA301471), were used for gene knockdown and protein detection to investigate the molecular mechanisms underlying the effects of LGALS9/galectin-9 treatment in KRAS mutant colon carcinoma cells.
Snippet: For RNA interference experiments, cells were precultured in a 12-well plates at a density of 1×105 cells/well for 24 h, after which siRNA transfections were performed with Dharmafect-2 (Dharmacon, T-2002–01) following the manufacturer's recommendations using a final concentration of 5 nM siRNA against BRAF (Origine, SR300470), RAF1/CRAF (Origene, SR303972), MAP2K1 (Origene, SR303761), BECN1 (Origene, SR305711) or ATG5 (Origene, SR306286). …Efficiency of knockdown of the target protein was evaluated (96 h after transfection) by western blotting (see below) using anti-BRAF (Santa Cruz Biotechnology, sc-5284), anti- RAF1/CRAF (Sigma Aldrich, R5773), anti-MEK1/2 (Cell Signaling Technology, 9122), anti-BECN1 (Origene, TA301471) and anti-ATG5 (Cell Signaling Technology, 9980), and appropriate secondary HRP-conjugated antibodies (Dako, P0447 and P0448)
WB
Showing 1-1 of 1 papers.
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