Srf Mouse shRNA Plasmid (Locus ID 20807)
SKU
TL502142
Srf - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
$1,013.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 20807 |
|---|---|
| Synonyms | AW049942; AW240594 |
| Vector | pGFP-C-shLenti |
| E. coli Selection | Chloramphenicol (34 ug/ml) |
| Mammalian Cell Selection | Puromycin |
| Format | Lentiviral plasmids |
| Components | Srf - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 20807). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
| RefSeq | BC051950, NM_020493, NM_020493.1, NM_020493.2 |
| UniProt ID | Q9JM73 |
| Summary | SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). Required for cardiac differentiation and maturation (PubMed:15169892).UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
SRF SUMOylation modulates smooth muscle phenotypic switch and vascular remodeling
Xu, Zhang, Chen et al
Nat Commun (2024) 15 (1), 6919 DOI: 10.1038/s41467-024-51350-5
Xu, Zhang, Chen et al
Nat Commun (2024) 15 (1), 6919 DOI: 10.1038/s41467-024-51350-5
Cited by 29
AI Summary: In this study, OriGene’s full-length mouse SRF plasmid (Cat#: TL502142) was used to generate Flag-tagged SRF constructs, enabling investigation of SRF SUMOylation and its role in smooth muscle cell phenotypic switching and vascular remodeling.
Snippet: 15 software (Olympus, Tokyo, Japan). Plasmids and transfection HA-SUMO1 was purchased in Addgene (Cambridge, MA, USA). To obtain Flag-SRF, full-length SRF (mouse, Locus ID 20807) was purchased from ORIGENE (CAT#: TL502142) and cloned into p3xFlag-Myc-CMV-24 vector. Various Flag-SRF mutations (K143R, K131R, K161R) were generated using SUMOylation prediction websites (SUMOplot, JASSA, and GPS-SUMO
SRF SUMOylation modulates smooth muscle phenotypic switch and vascular remodeling
Xu, Zhang, Pober et al
Res Sq (2023)
Xu, Zhang, Pober et al
Res Sq (2023)
Cited by 3
Snippet: 15 software (Olympus, Tokyo, Japan). Plasmids and transfection HA-SUMO1 was purchased in Addgene (Cambridge, MA, USA). To obtain Flag-SRF, full-length SRF (mouse, Locus ID 20807) was purchased from ORIGENE (CAT#: TL502142) and cloned into p3xFlag-Myc-CMV-24 vector. Various Flag-SRF mutations (K143R, K131R, K161R) were generated using SUMOylation prediction websites (SUMOplot, JASSA, and GPS-SUMO
Showing 1-2 of 2 papers.
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