Per2 Mouse shRNA Plasmid (Locus ID 18627)

Snippet: …Here, we identify that circadian regulator Period 2 (PER2) is significantly downregulated in BC and correlates with poor prognosis. PER2 functional deficiency mice ( PAS-B domain deletion, Per2 m/m ) display high susceptibility to DMBA/MPA-induced breast tumors. Tumor microenvironment (TME) profiling reveals that compared with Per2 wt tumors induced in Per2 wt mice, Per2 m/m tumors generated in Per2 m/m mice show reduced CD8⁺ T cells and M1-like macrophages infiltration and increased Foxp3 + Tregs and M2-like macrophages. Of note, reciprocal transplantation of Per2 m/m and Per2 wt tumors into Per2 wt or Per2 m/m mouse hosts reveals that systemic PER2 functional deficiency status not only boosts tumor growth but also promotes immunosuppression in TME. Consistently, Per2 knockdown in tumor cells enhances tumor cell proliferation and immune evasion in WT hosts. Mechanistically, PER2 knockdown leads to aberrant CLOCK transactivation, which upregulates the co-expression of HER2 and CD47, thereby leading to tumor aggressive growth with limited macrophage immune checkpoint surveillance. Collectively, our findings suggest that PER2 functional deficiency-driven CLOCK-HER2/CD47 axis promotes BC susceptibility and tumor immunosuppression, which provides a potential therapeutic target to mitigate CRD-associated BC risk and aggressiveness;- An shRNA plasmid targeting mPer2 (TL501620) was obtained from OriGene Technologies and used to generate a stable mPer2knockdown E0771 cell line. The pGFP-C

Snippet: fused with fluorophores as described before, kindly provided by Prof. Dominique Glauser (University of Fribourg, CH)48. Scramble Sh RNA vector used as a control for the in vitro silencing of Per2 (Origene cat #TR30021). Sh RNA Per2 Vector used for silencing Per2 in cell lines. (Origene cat #TL501620). Sequence: 5′-ATGAGCAGTGGCTCCAGCGGAAACGAGAA-3′. NIH 3T3 cells were co-transfected with e

Snippet: Dominique Glauser (University of Fribourg, CH) 48. -Scramble Sh RNA: vector used as a control for the in vitro silencing of Per2 (Origene cat #TR30021) -Sh RNA Per2: Vector used for silencing Per2 in cell lines. (Origene cat #TL501620)