Col12a1 Mouse shRNA Plasmid (Locus ID 12816)
SKU
TL500400
Col12a1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
$1,013.00
2 Weeks*
Specifications
| Product Data | |
| Locus ID | 12816 |
|---|---|
| Vector | pGFP-C-shLenti |
| E. coli Selection | Chloramphenicol (34 ug/ml) |
| Mammalian Cell Selection | Puromycin |
| Format | Lentiviral plasmids |
| Components | Col12a1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 12816). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
| RefSeq | NM_001290308, NM_007730, NM_007730.1, NM_007730.2, NM_001290308.1, BM963265 |
| UniProt ID | Q60847 |
| Summary | Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Temporal profiling of the breast tumour microenvironment reveals collagen XII as a driver of metastasis
Papanicolaou, Parker, Yam et al
Nat Commun (2022) 13 (1), 4587 DOI: 10.1038/s41467-022-32255-7
Papanicolaou, Parker, Yam et al
Nat Commun (2022) 13 (1), 4587 DOI: 10.1038/s41467-022-32255-7
Cited by 230
AI Summary: OriGene’s shColXII and shSCR control lentiviral GFP constructs (catalog number TL500400) were used in this study to genetically manipulate collagen XII expression, enabling the authors to investigate its role in organizing collagen I and promoting breast cancer metastasis.
Snippet: ection of short-hairpin constructs. Briefly, HEK293T cells were grown to 80% confluence in a 100 mm dish. Cells were transfected with 8 µg of shColXII or shSCR control lentiviral GFP constructs (OriGene, TL500400), together with 4.5 µg pMDLg/pRRE, 6.4 µg pRSV-Rev and 2.7 µg pMD.G third-generation packaging plasmids. Transfection was carried out in Opti-MEM reduced serum media using L
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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