CTCF Human shRNA Plasmid Kit (Locus ID 10664)
SKU
TL313675
CTCF - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
$1,013.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 10664 |
|---|---|
| Synonyms | CFAP108; FAP108; MRD21 |
| Vector | pGFP-C-shLenti |
| E. coli Selection | Chloramphenicol (34 ug/ml) |
| Mammalian Cell Selection | Puromycin |
| Format | Lentiviral plasmids |
| Components | CTCF - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 10664). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
| RefSeq | NM_001191022, NM_006565, NM_006565.1, NM_006565.2, NM_006565.3, NM_001191022.1, BC014267, BC014267.2, NM_001363916, NM_006565.4 |
| UniProt ID | P49711 |
| Summary | This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2010 |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Storage | Store at -20°C |
| Shipping | Ambient |
Reviews
Documents
Resources
Citations
Studying the Oncogenic and Epigenetic Impact of CTCF Loss of Heterozygosity and Zinc-Finger 1 Mutation in Breast Cancer
Lebeau
Thesis (2023)
Lebeau
Thesis (2023)
3D chromatin remodeling potentiates transcriptional programs driving cell invasion
Lebeau, Jangal, Zhao et al
Proc Natl Acad Sci U S A (2022) 119 (36), e2203452119 DOI: 10.1073/pnas.2203452119
Lebeau, Jangal, Zhao et al
Proc Natl Acad Sci U S A (2022) 119 (36), e2203452119 DOI: 10.1073/pnas.2203452119
Cited by 16
AI Summary: OriGene’s shCTCF (TL313675) and shCTL plasmids were used to achieve CTCF knockdown via transfection, enabling the study of how CTCF loss affects chromatin architecture and cell invasion.
Snippet: details. Transfection for shRNA CTCF Knockdown. Transfection is done following the Lipofectamine 3000 transfection reagent protocol (Invitrogen, L3000001) using 5 μg of shCTL or shCTCF plasmid from Origene (TL313675, locus ID 10664). Culture medium of 50% confluent cells was changed to Opti-MEM (Gibco, 11058-021) 30 min before transfection. Then, 6 h after transfection, culture medium was change
Showing 1-2 of 2 papers.
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