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MDM2 Human shRNA Plasmid Kit (Locus ID 4193)

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SKU
TL311529
MDM2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
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Other products for "MDM2 or NM_001145336"

Specifications
Specifications
Product Data
Locus ID 4193
Synonyms hdm2; HDMX; MGC5370; MGC71221
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components MDM2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 4193). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001145336, NM_001145337, NM_001145339, NM_001145340, NM_001278462, NM_002392, NM_006878, NM_006879, NM_006880, NM_006881, NM_006882, NM_002392.1, NM_002392.2, NM_002392.3, NM_002392.4, NM_002392.5, NM_001145337.1, NM_001145337.2, NM_001145339.1, NM_001145339.2, NM_001145340.1, NM_001145340.2, NM_001278462.1, NM_001145336.1, NM_006878.2, NM_006879.2, NM_006881.2, NM_006882.2, BC006795, BC065505, BC107414, BC148522, BC152384, BC152390, BC153117, NM_001367990, NM_001145337.3, NM_001145340.3, NM_001278462.2
UniProt ID Q00987
Summary This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. provided by RefSeq, Jun 2013
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
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file-alt Citations

Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53
de Queiroz, Efe, Guzman et al
Nat Commun (2024) 15 (1), 7132 DOI: 10.1038/s41467-024-51488-2
Cited by 14
AI Summary: OriGene products, specifically shRNAs against Mdm2 (cat.# TL311529) and Spry4 (cat.# HC108594), were used in this study to knock down these genes and investigate their roles in regulating cell migration, focal adhesion formation, and metastasis independently of p53.
Snippet: set of 4 shRNAs against Mdm2 (Origene, cat.#TL311529) or shRNA negative control ( of four shRNAs against Spry4 (OriGene, cat.#HC108594) or shRNA negative control (;hip between Mdm2, MdmX, and p53, there is substantial literature that describes p53-independent activities of Mdm2 and MdmX6,11. Mdm2 has been shown to regulate many cellular processes involved in tumorigenesis, such as regulation of chromatin state12 and DNA repair11, maintenance of cell survival and growth13,14, modulation of cell migration15,16 and promotion of metastasis17,18. …
FigureFigureFigure
Sprouty4 is required for Mdm2 regulation of invasion, focal adhesion formation and metastasis in cells lacking p53
Queiroz, Efe, Guzman et al
bioRxiv (2023)
Snippet: set of 4 shRNAs against Mdm2 (Origene, cat.#TL311529) or shRNA negative control ( of 4 shRNAs against Spry4 (OriGene, cat.#HC108594) or shRNA negative control (
MDM2 derived from dedifferentiated liposarcoma extracellular vesicles induces MMP2 production from preadipocytes
Casadei, Calore, Braggio et al
Cancer Res. (2019)
AI Summary: OriGene products contributed to this study by providing the non-targeting control vector plasmid and shRNA targeting endogenous human MDM2 transcript, which were used to manipulate MDM2 expression in experimental models.
Snippet: DNA plasmids, virus production and transduction Non-targeting control vector plasmid and shRNA targeting endogenous human MDM2 transcript were obtained from Origene. Both plasmids were packaged in Lenti-X 293T cell line
p16 Controls p53 Protein Expression through miR-dependent Destabilization of MDM2
Al-Khalaf, Aboussekhra
Mol. Cancer Res. (2018)
AI Summary: OriGene’s pGFP-C-shLenti-MDM2-shRNA was used in this study to specifically down-regulate MDM2 expression, enabling the investigation of p16INK4A’s role in regulating p53 protein levels.
Snippet: manufacturer. pGFP-C-shLenti-MDM2- shRNA (specific down-regulation of MDM2) (Origene), pIRES-Puro2 encoding FLAG-tagged human p16, and their corresponding control plasmids were used at 1 µg/ml for transfection using
p53 participates in the protective effects of ischemic post-conditioning against OGD-reperfusion injury in primary cultured spinal cord neurons
Li, Chen, Gao et al
Neurosci. Lett. (2016) 638, 129-134 DOI: 10.1016/j.neulet.2016.12.037
AI Summary: OriGene’s MDM2 shRNA (catalog number 17246) was used to inhibit MDM2 expression, enabling the study of the MDM2-p53 pathway’s role in the protective effects of ischemic post-conditioning against spinal cord neuron injury.
Snippet: 2.2. Construction of lentiviral vectors. We used lentiviral vectors containing MDM2 shRNA (MDM2 shRNA: 17246, Origene, Rockville, MD, USA) to inhibit the expression of MDM2, and a scrambled shRNA as a control of MDM2 shRNA. ... Cite SaveSaving
Showing 1-5 of 5 papers.
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