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TFE3 Human shRNA Plasmid Kit (Locus ID 7030)

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SKU
TL308868
TFE3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
2 Weeks*

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Other products for "TFE3 or NM_001282142"

Specifications
Specifications
Product Data
Locus ID 7030
Synonyms bHLHe33; RCCP2; RCCX1; TFEA
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components TFE3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 7030). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001282142, NM_006521, NM_006521.2, NM_006521.3, NM_006521.4, NM_006521.5, NM_001282142.1, BC026027, BC026027.1, BC001532, BM904206, NM_001282142.2
UniProt ID P19532
Summary This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
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file-alt Citations

Transcription Factor E3/Phosphatidylinositol 3-Kinase/Akt/Mammalian Target of Rapamycin Axis in Renal Cell Carcinoma Affects Tumor Microenvironment
Hwang, Kang, Kim et al
Am J Pathol (2024)
Cited by 8
AI Summary: OriGene products contributed to this study by providing the control lentivirus expressing scrambled shRNA (TR30021), lentiviral particles encoding TFE3 shRNAs (TL308868), the TurboFectin transfection reagent (TF81002), and packaging plasmids (TR30037), which were used to manipulate TFE3 expression in RCC cells and investigate its role in the PI3K/Akt pathway.
Snippet: 146 cells were transduced with a control lentivirus expressing scrambled shRNA (TR30021;OriGene Technologies, Rockville, MD) or lentiviral particles encoding four TFE3 shRNAs (TL308868;OriGene Technologies) (shTFE3-A: 5ʹ-CAGAAGAAAGACAATCACAACCTAATTGA-3ʹ, shTFE3-B: 5ʹ-TCAGCCTGGAGTCCAGTTACAATGTAGAA-3ʹ, shTFE3-C: 5ʹ-CGCTCCAAAGACCTGGAGAGCCGGCAGCG-3ʹ, and shTFE3-D: 5ʹ-AGCCOGTTCAGCATGGAAGAGGAGTCCTG-3ʹ), according to the manufacturer’s guidelines. Lentiviruses were produced by transient transfection of HEK293 cells (RRID: CVCL_0045) using TurboFectin transfection reagent (TF81002;OriGene Technologies), lentivirus vectors, and packaging plasmids (TR30037;OriGene Technologies). …
Showing 1-1 of 1 papers.
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