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FTO Human shRNA Plasmid Kit (Locus ID 79068)

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SKU
TL308064
FTO - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
  $1,013.00
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Specifications
Specifications
Product Data
Locus ID 79068
Synonyms ALKBH9; BMIQ14; GDFD
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Components FTO - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 79068). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001080432, NM_001080432.1, NM_001080432.2, BC001284, BC003583, BC030798, BC132892, BC137091, BC148442, BC153032, NM_001363905, NM_001363988, NM_001363891, NM_001363894, NM_001363896, NM_001363897, NM_001363898, NM_001363899, NM_001363900, NM_001363901, NM_001363903, NR_156761, NM_001080432.3
UniProt ID Q9C0B1
Summary This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. provided by RefSeq, Jul 2011
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
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file-alt Citations

MicroRNA-33a-5p alleviates cervical cancer by regulating PDE4B through FTO-mediated m6A demethylation
Sun, Huang, Xu et al
Cytotechnology (2026) 78 (3) DOI: 10.1007/s10616-026-00970-4
AI Summary: OriGene products, including sh-NC (TL301128V), sh-FTO#1 (TL308064), sh-FTO#2 (TL308064V), sh-PDE4B#1 (TL310546), and sh-PDE4B#2 (TL310546V), were used in this study to specifically knock down FTO and PDE4B gene expression, enabling the investigation of their roles in cervical cancer regulation by microRNA-33a-5p.
Snippet: the sh-NC (cat. no. TL301128V), sh-FTO#1 (cat. no. TL308064), sh-FTO#2 (cat. no. TL308064V), sh-PDE4B#1 (cat. no. TL310546), and sh-PDE4B#2 (cat. no. TL310546V) were bought from ORIGENE (MD, USA)
FTO-mediated cytoplasmic m6Am demethylation adjusts stem-like properties in colorectal cancer cell
Relier, Ripoll, Guillorit et al
Nat Commun (2021) 12 (1), 1716 DOI: 10.1038/s41467-021-21758-4
Cited by 182
AI Summary: In this study, OriGene products, specifically the FTO shRNA (OriGene, #TL313869) and FTO overexpression plasmid (OriGene, #RC208934), were used to modulate FTO expression in colorectal cancer cell lines, enabling the authors to investigate the role of FTO-mediated m6Am demethylation in regulating cancer stem cell properties.
FigureFigureFigure
FTO-Dependent N6-Methyladenosine Modifications Inhibit Ovarian Cancer Stem Cell Self-Renewal by Blocking cAMP Signaling
Huang, Wang, Kandpal et al
Cancer Res. (2020) 80 (16), 3200-3214 DOI: 10.1158/0008-5472.CAN-19-4044
AI Summary: OriGene’s lentiviral vector-based shRNAs targeting human FTO (shFTO-1, shFTO-2) and non-targeting control shRNA were used to manipulate FTO expression in ovarian cancer stem cells, enabling the study of FTO’s role in regulating stemness and tumorigenesis.
Snippet: … The lentiviral vector based shRNAs targeting human FTO (shFTO-1, shFTO-2) and non-targeting control shRNA were purchased from Origene Technologies (Rockville, MD). … The lentiviral vector based shRNAs targeting human FTO (shFTO-1, shFTO-2) and non-targeting control shRNA were purchased from Origene Technologies (Rockville, MD). …
FTO-mediated cytoplasmic m6Am demethylation adjusts stem-like properties in colorectal cancer cell
Relier, Ripoll, Guillorit et al
bioRxiv (2020)
Cited by 6
Snippet: … HAL Id: hal-03000860 https://hal.archives-ouvertes.fr/hal-03000860 Preprint submitted on 12 Nov 2020 HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not;FTO mediated m6Am demethylation takes place in the cytoplasm. (a) FTO localizes both in nucleus and cytoplasm. Immunofluorescence staining of FTO (green) in CRC1 and SW620 cell lines show presence of FTO in cytoplasm and in nuclear speckles. …(c) FTO silencing increases cytoplasmic m6Am level. …(d) FTO relocalizes to the cytoplasm during tumorigenesis. FTO expression and localization was evaluated by IHC on TMA from CRC patient. Then, nuclear and cytoplasmic level of FTO were quantified. … Bar plots represents mean +/- S.E.M of H-score based on nuclear and cytoplasmic intensity of FTO staining. …
The m6A eraser FTO facilitates proliferation and migration of human cervical cancer cells
Zou, Dong, Li et al
Cancer Cell Int. (2019) 19, 321 DOI: 10.1186/s12935-019-1045-1
Cited by 152
AI Summary: OriGene products pGFP-C-shFTO and pGFP-C-shNC were used to knock down FTO expression in cervical cancer cell lines, enabling the study of FTO's role in cell proliferation and migration.
Snippet: Plasmid construction and transfections. The pGFP-C-shFTO and pGFP-C-shNC were purchased from Origene technologies (Rockville, MD). The pcDNA3.1_FTO and pcDNA3.1_mutFTO constructs were kindly provided by Dr. Jianjun Chen Cached
FigureFigureFigure
R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
Su, Dong, Li et al
Cell (2018) 172 (1-2), 90-105.e23 DOI: 10.1016/j.cell.2017.11.031
AI Summary: In this study, OriGene's shFTO-2 shRNA was used to specifically knock down FTO expression, helping to elucidate the role of FTO in R-2HG’s anti-tumor activity.
Snippet: pCDH-puro-MYC was purchased from Addgene (Cambridge, MA). shRNAs against FTO (shFTO-1) and YTHDF2 (shYTHDF2-1 and shYTHDF2-2) were inserted into pLKO.1 vector. The other shRNA against FTO (shFTO-2) were purchased from OriGene
Figure
Showing 1-6 of 8 papers.
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