Tdg Mouse shRNA Plasmid (Locus ID 21665)
SKU
TG502249
Tdg - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
$883.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 21665 |
|---|---|
| Synonyms | E130317C12Rik; JZA-3; Jza1 |
| Vector | pGFP-V-RS |
| E. coli Selection | Kanamycin |
| Format | Retroviral plasmids |
| Components | Tdg - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 21665). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
| RefSeq | BC010315, BC085470, BC085471, NM_011561, NM_172552, NM_001358517, NM_011561.1, NM_011561.2, NM_172552.1, NM_172552.2, NM_172552.3, NM_172552.4 |
| UniProt ID | P56581 |
| Summary | DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine (PubMed:21817016). Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC (PubMed:21722948). Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Shipping | Ambient |
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