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p53 (TP53) Human shRNA Plasmid Kit (Locus ID 7157)

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SKU
TG320558
TP53 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
  $883.00
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Specifications
Specifications
Product Data
Locus ID 7157
Synonyms BCC7; BMFS5; LFS1; P53; TRP53
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components TP53 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 7157). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_000546, NM_001126112, NM_001126113, NM_001126114, NM_001126115, NM_001126116, NM_001126117, NM_001126118, NM_001276695, NM_001276696, NM_001276697, NM_001276698, NM_001276699, NM_001276760, NM_001276761, NM_000546.1, NM_000546.2, NM_000546.3, NM_000546.4, NM_000546.5, NM_001126116.1, NM_001126117.1, NM_001126115.1, NM_001126114.1, NM_001126114.2, NM_001126113.1, NM_001126113.2, NM_001126112.1, NM_001126112.2, NM_001276696.1, NM_001276695.1, NM_001126118.1, NM_001276760.1, NM_001276761.1, NM_001276698.1, NM_001276699.1, NM_001276697.1, BC003596, BC003596.1, NM_000546.6, NM_001276761.2, NM_001276699.2, NM_001276696.2, NM_001276698.2, NM_001276697.2, NM_001276695.2, NM_001276760.2
UniProt ID P04637
Summary This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). provided by RefSeq, Dec 2016
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
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Documents
Resources

file-alt Citations

COUP-TFII Overexpression Inhibits Cell Proliferation and Invasion via Increased Expression of p53 and PTEN and Decreased Akt Phosphorylation in Human Colorectal Cancer SNU-C4 Cells
Yun, Park
Anticancer Res. (2020) 40 (2), 767-777 DOI: 10.21873/anticanres.14008
Cited by 3
AI Summary: OriGene's p53 shRNA plasmid (pGFP-V-RS-p53 shRNA) was used to knock down p53 expression in SNU-C4 cells, helping to elucidate the role of p53 in COUP-TFII-mediated inhibition of cell proliferation and invasion.
Snippet: COUP-TFII-SNU-C4 cells were transfected with 2 μg of NC shRNA or p53 shRNA plasmid (pGFP-V-RS-p53 shRNA;Origene, Rockville, MD, USA) using lipofectamine 2000 according to the manufacturer's procedure, and treated with G418 (800 μg/ml) and puromycin
p53-Dependent and -Independent Responses of Cells Challenged by Photosensitization
de P Abrantes, Dias, de Souza-Pinto et al
Photochem. Photobiol. (2018)
AI Summary: OriGene’s p53 shRNA plasmid (TG320558) was used to generate HEK293T cells with reduced p53 expression, enabling the study of how p53 influences cellular responses to photosensitization-induced stress and cell death.
Snippet: HEK293T scramble (sc)] were obtained by using Lipofectamine 2000 (Invitrogen) with plasmids expressing p53 shRNA or scrambled shRNA (TG320558, OriGene), followed by selection of clones in complete medium containing 0.5-2 μg/mL puromycin (Gibco)
Avian Reovirus Protein p17 Functions as a Nucleoporin Tpr Suppressor Leading to Activation of p53, p21 and PTEN and Inactivation of PI3K/AKT/mTOR and ERK Signaling Pathways
Huang, Chiu, Liao et al
PLoS ONE (2015) 10 (8), e0133699 DOI: 10.1371/journal.pone.0133699
Cited by 47
AI Summary: OriGene products contributed to this study by providing all the shRNAs and scrambled negative shRNA used for gene knockdown experiments to investigate the roles of Tpr, p53, PTEN, and other related proteins.
Snippet: The AKT DN mutant was kindly provided by Professor Shih, National Pingtung University of Science and Technology, Taiwan. In the present study, all shRNAs and scrambled negative shRNA (Table 2) purchased from OriGene Co. ... Cite SaveSaving
FigureFigureFigure
Gene expression profiles resulting from stable loss of p53 mirrors its role in tissue differentiation
Couture, Lombardi, Davis et al
PLoS One (2013) 8 (11), e82494 DOI: 10.1371/journal.pone.0082494
Cited by 6
AI Summary: OriGene products contributed to this study by providing the p53 shRNA (OriGene, Cat# TL315966) used for stable knockdown of p53 in C2C12 cells, enabling the authors to assess gene expression changes resulting from long-term p53 loss.
FigureFigureFigure
Deacetylation of p53 induces autophagy by suppressing Bmf expression
Contreras, Mebratu, Delgado et al
J. Cell Biol. (2013) 201 (3), 427-37 DOI: 10.1083/jcb.201205064
Cited by 48
AI Summary: OriGene products contributed to this study by providing the human Bmf shRNA plasmid (Cat# TG313148), which was used to knock down Bmf expression and assess its role in IFN-γ-induced autophagy.
Increased geranylgeranylated K-Ras contributes to antineoplastic effects of farnesyltransferase inhibitors
Hall
Thesis (2012)
Snippet: Scrambled and TP53 (TG320558) shRNA pGFP-V-RS retroviral plasmids were purchased from OriGene Technologies, Inc (Rockville, MD)
Showing 1-6 of 6 papers.
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