Pld1 Rat shRNA Plasmid (Locus ID 25096)

SKU
TF711124
Pld1 - Rat, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
  $883.00
In Stock*
Specifications
Specifications
Product Data
Locus ID 25096
Synonyms Pld1a; Pld1b; Plda; Pldb
Vector pRFP-C-RS
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Components Pld1 - Rat, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 25096). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_030992, NM_030992.1
UniProt ID P70496
Summary ubiquitous enzyme that hydrolyzes phosphatidylcholine to phosphatidic acid (PA) and choline RGD, Feb 2006
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage Store at -20°C
Shipping Ambient
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Documents
Resources

file-alt Citations

Choline Is an Intracellular Messenger Linking Extracellular Stimuli to IP3-Evoked Ca2+ Signals through Sigma-1 Receptors
Brailoiu, Chakraborty, Brailoiu et al
Cell Rep (2019) 26 (2), 330-337.e4 DOI: 10.1016/j.celrep.2018.12.051
AI Summary: OriGene products, specifically the Rabbit anti-Sig-1R antibody (Cat# TA302033) and Mouse anti-GFP antibody (Cat# TA150041), were used in this study to detect Sigma-1 receptors and GFP-tagged proteins, enabling the investigation of choline’s role as an intracellular messenger acting through Sigma-1 receptors.
Snippet: 800CW-conjugated goat anti-rabbit IgG (1:10,000) LI-COR, Lincoln, NE Cat# 926-32211 IRDye 680-conjugated goat anti-mouse IgG (1:10,000) LI-COR Cat# 926-32220 Rabbit anti-Sig-1R (1:200) OriGene, Rockville, MD Cat# TA302033 Rabbit anti-Sig-1R (2 μg/mL) AbCam, Cambridge, UK Cat# 53852 Mouse anti-GFP (1:2000) OriGene Cat# TA150041 Mouse anti-PLD1 (1:200) Santa Cruz B;... 800CW-conjugated goat anti-rabbit IgG (1:10,000) LI-COR, Lincoln, NE Cat# 926-32211 IRDye 680-conjugated goat anti-mouse IgG (1:10,000) LI-COR Cat# 926-32220 Rabbit anti-Sig-1R (1:200) OriGene, Rockville, MD Cat# TA302033 Rabbit anti-Sig-1R (2 μg/mL) AbCam, Cambridge, UK Cat# 53852 Mouse anti-GFP (1:2000) OriGene Cat# TA150041 Mouse anti-PLD1 (1:200) Santa Cruz B;IRDye 680-conjugated goat anti-mouse IgG (1:10,000), LI-COR, Cat# 926-32220. Rabbit anti-Sig-1R (1:200), OriGene, Rockville, MD, Cat# TA302033. … Mouse anti-GFP (1:2000), OriGene, Cat# TA150041;Rabbit anti-Sig-1R (1:200) OriGene, Rockville, MD Cat# TA302033 Rabbit anti-Sig-1R (2 μg/mL) AbCam, Cambridge, UK Cat# 53852 Mouse anti-GFP (1:2000) OriGene Cat# TA150041
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