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pRFP-C-RS shRNA Vector

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SKU
TR30014
HuSH shRNA RFP Retroviral Cloning Vector (pRFP-C-RS Vector), 5ug
  $670.00
In Stock*

Frequently Bought Together (1)

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5 x 200 ul
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Specifications
Specifications
Product Data
Control Type Negative
Fluorescent Label RFP
Mammalian Cell Selection Puromycin
Viral Packaging Retroviral
Features
  • The HuSH pRFP-C-RS plasmid vector was created with an integrated turboRFP element to readily verify transfection efficiency. It incorporates both a chloramphenicol and puromycin resistance elements for greater selection capabilities. The pRFP-C-RS plasmid is also ideal for monitoring the dual-gene knockdown experiments when used alongside pGFP-V-RS expression plasmid.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Components 1 vial of 5 ug lyophilized pRFP-C-RS shRNA cloning vector
Quality Control This vector was sequence verified and functionally validated.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
Resources

file-alt Citations

Neutrophil Gelatinase-Associated Lipocalin Protects Acinar Cells From Cerulein-Induced Damage During Acute Pancreatitis
Bhatia, Muniyan, Thompson et al
Pancreas (2020) 49 (10), 1297-1306 DOI: 10.1097/MPA.0000000000001690
AI Summary: OriGene products contributed to this study by providing the 24p3R overexpression construct (cat. no. MR226001) and the 24p3R shRNA plasmid in pRFP-C-RS vector (cat. no. TR30014), which were used to manipulate 24p3R expression and investigate its role in acinar cell response to NGAL during acute pancreatitis.
Snippet: …MR226001, Origene, Rockville, Md) in HEK293 cells. …TR30014, Origene) and used for transient silencing of 24p3R in 266.6 cells using the Turbofect transfection reagent (cat. …
HECTD1 regulates the expression of SNAIL: Implications for epithelial‑mesenchymal transition
Wang, De Geyter, Jia et al
Int J Oncol (2020)
Cited by 24
AI Summary: OriGene products, specifically the HECTD1 shRNA constructs (cat. no. TF304134) and the shRNA control (cat. no. TR30014), were used to knock down HECTD1 expression, enabling the study of its role in regulating SNAIL stability and epithelial-mesenchymal transition in cervical cancer cells.
Snippet: no. TF304134;OriGene Technologies, Inc., which consists of 4 shRNAs against HECTD1, cat. nos no. TR30014;OriGene Technologies, Inc.). The RFP expression cassettes in the shRNA constructs were excised with XhoI/BglII and blunt‑end ligation
Aberrant KIF20A Expression Is Associated with Adverse Clinical Outcome and Promotes Tumor Progression in Prostate Cancer
Zhang, Chai, Shen et al
Dis. Markers (2019) 2019, 4782730 DOI: 10.1155/2019/4782730
AI Summary: OriGene's KIF20A human shRNA plasmid (TG311916) and HuSH shRNA RFP Cloning Vector (TR30014) were used to knock down KIF20A expression in prostate cancer cells, enabling the study of its role in tumor progression.
Snippet: To knock down KIF20A in PCa cells, C4-2 cells were seeded in six-well plates and then a prepared plasmid was added (OriGene, KIF20A human shRNA plasmid, TG311916, HuSH shRNA RFP Cloning Vector, TR30014, USA) that was mixed with a Transfection Reagent Cached
FigureFigureFigure
KIF20A Affects the Prognosis of Bladder Cancer by Promoting the Proliferation and Metastasis of Bladder Cancer Cells
Shen, Yang, Zhang et al
Disease Markers (2019) 2019, 1-9 DOI: 10.1155/2019/4863182
AI Summary: OriGene products, specifically the KIF20A human shRNA plasmid (CAT#: TG311916) and the HuSH shRNA RFP cloning vector (CAT#: TR30014), were used to knock down KIF20A expression in bladder cancer cell lines, enabling the study of its role in cell proliferation and metastasis.
Snippet: 2.8. In Vitro Transfection. The following shRNA plasmids were used in this study for in vitro transfection: KIF20A human shRNA plasmid (CAT#: TG311916, OriGene) and HuSH shRNA RFP cloning vector (CAT#: TR30014, OriGene). The cells were transfected with liposomes Cached
In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus
Rosin, Kurrasch
J Neuroinflammation (2018) 15 (1), 181 DOI: 10.1186/s12974-018-1213-6
AI Summary: In this study, the OriGene pRFP-C-RS expression vector (TR30014) was used as one of the plasmids for in utero electroporation to label cells in embryonic mouse brains, enabling the investigation of how this technique affects microglia morphology and gene expression.
Snippet: In addition, the pCIC-Ascl1 expression vector, which contains a β-actin promoter/CMV enhancer upstream of the Ascl1 sequence and an IRES-mCherry cassette, and the pRFP-C-RS expression vector (TR30014, OriGene), which contains a CMV promoter and Cached
FigureFigureFigure
Calcium release mediated by redox-sensitive RyR2 channels has a central role in hippocampal structural plasticity and spatial memory
More, Bruna, Lobos et al
Antioxid. Redox Signal. (2018)
AI Summary: OriGene products, specifically the pRFP-C-RS and pRFP-C-RS (TR30014) vectors and plasmids containing a noneffective 29-mer scrambled shRNA cassette (TR30013 and TR30015), were used for shRNA-mediated down-regulation of RyR2 expression to investigate its role in hippocampal structural plasticity and spatial memory.
Snippet: …The pRFP-C-RS and pRFP-C-RS (TR30014) vectors and plasmid containing a noneffective 29-mer scrambled shRNA cassette (TR30013 and TR30015) were from Origene (Rockville, MA). …
Showing 1-6 of 8 papers.
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