NEK2 (NM_002497) Human Recombinant Protein

SKU TP762163

Purified recombinant protein of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, Met1-Tyr240, with N-terminal His tag, expressed in E.coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region(Met1-Tyr240) of NEK2
• Tag: N-His
• Predicted MW: 27.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_002488
• Locus ID: 4751
• UniProt ID: P51955
• Cytogenetics: 1q32.3
• RefSeq Size: 2161
• RefSeq ORF: 1335
• Synonyms: HsPK21; NEK2A; NLK1; PPP1R111; RP67
• Summary: This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
• Protein Families: Druggable Genome, Protein Kinase