OPN5 Human Recombinant Protein, Synthetic Nanodisc
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Tag | C-Flag & Strep Tag |
| Predicted MW | 39.7 kDa |
| Concentration | Please refer to the Certificate of Analysis (COA) for the lot-specific concentration before lyophilization. |
| Buffer | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
| Reconstitution Method | 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube. 2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000 3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL) 4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample. |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. |
| Stability | After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing. |
| Shipping | Ambient |
| Reference Data | |
| UniProt ID | Q6U736 |
| Synonyms | GPR136, GRP136, PGR12, TMEM13 |
| Summary | Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this family member may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2010 |
| Protein Categories | GPCR, Membrane Proteins |
| Protein Families | Druggable Genome, Transmembrane |
Reviews
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