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ACHE Human Recombinant Protein, Synthetic Nanodisc

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SKU
TP728576
Recombinant Human ACHE full length protein-Synthetic Nanodisc, 10 ug
Size:
10 ug 100 ug
  $1,265.00
8 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Tag C-Flag & Strep Tag
Predicted MW 54.7 kDa
Concentration Please refer to the Certificate of Analysis (COA) for the lot-specific concentration before lyophilization.
Buffer Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments.
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Storage Store at -20°C to -80°C for 12 months in lyophilized form.
Stability After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing.
Shipping Ambient
Reference Data
UniProt ID Q04844
Synonyms ACHRE, CMS1D, CMS1E, CMS2A, CMS4A, CMS4B, CMS4C, FCCMS, SCCMS
Summary Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. provided by RefSeq, Sep 2009
Protein Categories Intracellular Proteins, Membrane Proteins, Nervous system Diseases
Protein Families Ion Channels: Cys-loop Receptors
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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