CD27 Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Human Fc Tag |
| Predicted MW | The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide.The apparent molecular mass of CD27-hFc is approximately 55-70 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Buffer | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization |
| Reconstitution Method | 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube. 2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000 3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL) 4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample. |
| Preparation | Affinity purification |
| Recommended Dilution | ELISA |
| Storage | Store the lyophilized protein at -20°C. After reconstitution, store the protein at -80°C for 12 months. Avoid repeated freezing and thawing. |
| Stability | 12 months from date of despatch |
| Shipping | Ambient |
| Reference Data | |
| Locus ID | 939 |
| UniProt ID | P26842 |
| Synonyms | CD27;TNFRSF7;S152;T14;Tp55 |
| Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| Protein Categories | CD Markers, Immune system diseases, Membrane Proteins |
| Protein Families | ELISA |
| Protein Pathways | Cytokine-cytokine receptor interaction |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CD27" proteins (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH304252 | CD27 MS Standard C13 and N15-labeled recombinant protein (NP_001233) | 10 ug |
$3,360.00
|
|
| LC400496 | Lyophilized CD27 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400496 | Transient overexpression lysate of CD27 molecule (CD27) (as WB positive control) | 100 ug |
$436.00
|
|
| TP304252 | Recombinant protein of human CD27 molecule (CD27), 20 µg | 20 ug |
$737.00
|
|
| TP700286 | Purified recombinant protein of human CD27 molecule (CD27), with C-terminal DDK/His tag, expressed in human cells, 20 µg | 20 ug |
$900.00
|
|
| TP723936 | Recombinant human CD27 protein with C-terminal mouse Fc and 6×His tag | 100 ug |
$905.00
|
|
Citations
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