CD27 Human Recombinant Protein

SKU
TP724010
Recombinant human CD27 protein with C-terminal human Fc tag
  $905.00
4 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
CD27(Ala20-Arg191) hFc(Glu99-Ala330)
Tag C-Human Fc Tag
Predicted MW The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide.The apparent molecular mass of CD27-hFc is approximately 55-70 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Preparation Affinity purification
Recommended Dilution ELISA
Storage Store the lyophilized protein at -20°C.
After reconstitution, store the protein at -80°C for 12 months.
Avoid repeated freezing and thawing.
Stability 12 months from date of despatch
Shipping Ambient
Reference Data
Locus ID 939
UniProt ID P26842
Synonyms CD27;TNFRSF7;S152;T14;Tp55
Summary The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Protein Categories CD Markers, Immune system diseases, Membrane Proteins
Protein Families ELISA
Protein Pathways Cytokine-cytokine receptor interaction
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "CD27" proteins (6)
SKU Description Size Price
PH304252 CD27 MS Standard C13 and N15-labeled recombinant protein (NP_001233) 10 ug
$3,360.00
LC400496 Lyophilized CD27 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400496 Transient overexpression lysate of CD27 molecule (CD27) (as WB positive control) 100 ug
$436.00
TP304252 Recombinant protein of human CD27 molecule (CD27), 20 µg 20 ug
$737.00
TP700286 Purified recombinant protein of human CD27 molecule (CD27), with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP723936 Recombinant human CD27 protein with C-terminal mouse Fc and 6×His tag 100 ug
$905.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products