CD14 (NM_000591) Human Recombinant Protein

SKU TP723883

Purified recombinant protein of Human CD14 molecule (CD14), transcript variant 1

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human CD14, the region of Thr20-Cys352, from gene Accession# NM_000591
• Tag: Tag Free
• Predicted MW: 35.8 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS, pH 7.2
• Bioactivity: The ED50 is 100-400 ng/ml, corresponding to a specific activity of 1.0-2.5 x 104 units/mg, determined by production of IL-8 from THP-1 cells trated with LPS (2 ng/ml) and CD14.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_000582
• Locus ID: 929
• UniProt ID: P08571
• Cytogenetics: 5q31.3
• RefSeq Size: 1623
• RefSeq ORF: 1125
• Summary: The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway