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CD47 Human Recombinant Protein, Synthetic Nanodisc

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SKU
TP721411
Human CD47 full length protein-synthetic nanodisc
Size:
10 ug 100 ug
  $1,395.00
3 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
C-terminal Flag tagged overexpression cDNA clone
Tag C-Flag Tag
Predicted MW The human full length CD47 protein has a MW of 35.2 kDa
Purity The purity of the protein is determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments.
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Preparation Affinity purification
Storage Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Stability After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing.
Shipping Ambient
Reference Data
Locus ID 961
UniProt ID Q08722
Synonyms IAP; MER6; OA3
Summary A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Protein Categories ADC Targets, CAR-T Targets, CD Markers, Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways ECM-receptor interaction
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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